Fluidic device and method for separating biomolecules
The present disclosure provides a device for separating biomolecules comprising a substrate having a planar surface, nanowires disposed on at least a portion of the planar surface, and a fluid chamber formed to include at least a portion of the nanowires.
1. A fluidic device for separating a biomolecule comprising:
a substrate having a planar surface;
nanowires disposed on at least a portion of the planar surface; and
a cover having a bonding surface and a recess surrounded by the bonding surface, the bonding surface being in intimate contact with a portion of the planar surface of the substrate,
wherein the planar surface of the substrate and the recess of the cover define a fluid chamber including at least a portion of the nanowires.
2. The fluidic device of claim 1 , wherein a catalyst layer for nanowire growth is formed on a planar surface of the substrate, and the nanowire is formed on the catalyst layer.
3. The fluidic device of claim 1 , wherein one end of the nanowire is embedded in a planar surface of the substrate.
4. The fluidic device of claim 1 , comprising an inlet for introducing a solution into the fluidic chamber and an outlet for discharging a solution from the fluidic chamber.
5. The fluidic device of claim 1 , wherein the cover has an inlet for introducing a solution into the fluid chamber and an outlet for discharging a solution from the fluid chamber.
6. The fluidic device of claim 5 , wherein a surface of the recess of the cover is formed with a microstructure for agitating the flow of the solution.
7. The fluidic device of claim 1 , wherein the biomolecule comprises RNA.
8. The fluidic device of claim 1 , wherein the biomolecule comprises an extracellular vesicle.
9. A method of separating a biomolecule comprising:
providing a fluidic device comprising a substrate having a planar surface;
nanowires disposed on at least a portion of the planar surface;
a cover having a bonding surface and a recess surrounded by the bonding surface, the bonding surface being in intimate contact with a portion of the planar surface of the substrate,
wherein the planar surface of the substrate and the recess of the cover define a fluid chamber including at least a portion of the nanowires; and
introducing a solution comprising the biomolecule into the fluidic device.
10. The method of claim 9 , wherein the biomolecule comprises at least one of a cell, a virus, and a bacterium.
11. The method of claim 10 , wherein the biomolecule comprises an extracellular vesicle.
12. The method of claim 11 , further comprising introducing a cell lysate into the fluidic device.
13. The method of claim 12 , further comprising: draining the cell lysate introduced into the fluidic device from the fluidic device; and measuring nucleic acids contained in the cell lysate drained from the fluidic device.
14. The method of claim 13 , wherein the nucleic acid comprises RNA.