Method of creating human pluripotent stem cell derived brain pericyte-like cells
A population of brain pericyte-like cells, wherein the cells express pericyte markers but do not express ACTA2 and wherein the cells are generated from hPSCs, is disclosed herein.
1. A method of creating a population of brain pericyte-like cells, wherein the cells express pericyte markers but do not express α-smooth muscle actin (α-SMA)and wherein the cells are generated from human pluripotent stem cells (hPSC), comprising the steps of
a. culturing hPSC in culture medium supplemented with CHIR99021, SB431542, fibroblast growth factor 2, and dorsomorphin for about 15 days to produce p75-nerve growth factor receptor (NGFR)+ human natural killer-1 (HNK-1)+ neural crest stem cells (NCSCs),
b. sorting p75-NGFR + cells and re-plating the p75-NGFR + cells to produce an enriched population of p75-NGFR + NCSCs,
c. culturing the cells of step (b) in medium with an addition of serum for about 11 days, and
d. isolating cells that express pericyte markers calponin 1 (CNN1), neuron-glial antigen 2 (NG2), and platelet-derived growth factor receptor beta (PDGFRB) but do not express α-SMA to produce population of brain pericyte-like cells.
2. The method of claim 1 , wherein the NCSCs of step (b) are p75-NGFR + HNK-1 + AP-2 + NCCs.
3. The method of claim 1 , wherein the population of cells express one or more transcripts of pericyte markers selected from the group consisting of chondroitin sulfate proteoglycan 4 (CSPG4), PDGFRB, CNN1, transgelin (TAGLN), alanyl aminopeptidase (ANPEP), T-box transcription factor 18 (TBX18), ATP binding cassette subfamily C member 9 (ABCC9) and potassium inwardly rectifying channel subfamily J member 8 (KCNJ8).
4. The method of claims 1 , wherein the method produces the brain pericyte-like cell population that is at least 90% NG2 + PDGFRB + .