Antibodies to aripiprazole haptens and use thereof
Disclosed is an antibody which binds to aripiprazole, which can be used to detect aripiprazole in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of aripiprazole, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.
1. A method of producing an antibody which binds to aripiprazole, the method comprising:
(i) selecting a host for antibody production; and
(ii) inoculating the host with a conjugate of a compound selected from the group consisting of:
wherein m is 1, 2, 3, 4, or 5, and n is 1, 2, 3, 4, or 5,
and an immunogenic carrier, wherein the host produces an antibody which binds to aripiprazole.
2. A method of producing a hybridoma cell line capable of producing a monoclonal antibody that binds to aripiprazole, the method comprising:
(i) selecting a host for antibody production;
(ii) inoculating the host with a conjugate of a compound selected from the group consisting of:
wherein m is 1, 2, 3, 4, or 5, and n is 1, 2, 3, 4, or 5,
and an immunogenic carrier;
(iii) fusing a cell line from said inoculated host with a continuously dividing cell to create a fused cell capable of producing a monoclonal antibody which binds to aripiprazole; and
(iv) cloning the fused cell so as to obtain a hybridoma cell line.
3. The method of claim 1 , wherein the immunogenic carrier is a protein.
4. The method of claim 3 , wherein the protein is keyhole limpet hemocyanin, bovine thyroglobulin, or ovalbumin.
5. The method of claim 2 , wherein the immunogenic carrier is a protein.
6. The method of claim 5 , wherein the protein is keyhole limpet hemocyanin, bovine thyroglobulin, or ovalbumin.
7. The method of claim 1 , wherein the antibody is a monoclonal antibody.
8. The method of claim 1 , further comprising isolating the antibody or binding fragment thereof.
9. The method of claim 2 , wherein the hybridoma cell line produces a monoclonal antibody that binds to aripiprazole.
10. The method of claim 2 , further comprising isolating the hybridoma cell line.
11. The method of claim 10 , further comprising producing a monoclonal antibody that binds to aripiprazole.