IP Library Granted Patent US 11,854,669
Granted Patent B1
US 11,854,669 · App. 16/512,577 · Granted Dec 26, 2023

Synthetic nucleic acids for information storage and transmission

Inventors: George Bachand (Albuquerque, NM); Marlene Bachand (Albuquerque, NM); Andrew Gomez (Edgewood, NM)
Assignee: National Technology & Engineering Solutions of Sandia, LLC
G16B50/40H04L9/0866
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Quick Facts
Patent No.
US 11,854,669
App. No.
16/512,577
Granted
Dec 26, 2023
Kind
B1
Abstract

The present invention relates to method for storing and transmitting information by employing a nucleic acid construct. The nucleic acid construct can include a lock region; a translation key region that corresponds to the identity of a key; and a message region including a nucleic sequence that corresponds to an encrypted message.

Claims (47)

1. A method for storing and transmitting information, the method comprising:

converting each character in an information format into a triplet or quadruplet codon by employing a key, thereby generating an encrypted message represented by a nucleic acid or a complement thereof;

synthesizing a nucleic acid construct comprising a lock region comprising a nucleic acid sequence with a primer binding region or a complement thereof, a translation key region comprising a nucleic acid sequence that corresponds to an identity of the key or a complement thereof, and a message region having a nucleic acid sequence comprising the encrypted message or a complement thereof;

relaying the nucleic acid sequence of the lock region to a recipient and the key corresponding to the translation key region; and

placing the nucleic acid construct on an object to be transmitted to the recipient;

wherein the synthesizing step further comprises:

inserting a start codon indicating the beginning of the message region, and

inserting a stop codon indicating the end of the message region.

2. The method of claim 1 , wherein the synthesizing step further comprises:

sectioning the nucleic acid construct, or a complement thereof, into a plurality of sections; and

assembling each section by employing one or more of an exonuclease, a polymerase, and/or a ligase.

3. The method of claim 1 , wherein the synthesizing step further comprises:

inserting the nucleic acid construct into a vector; and

expressing the vector.

4. The method of claim 1 , further comprising:

degrading the nucleic acid construct by employing a nuclease.

5. The method of claim 1 , further comprising:

amplifying the nucleic acid construct by employing a polymerise, thereby generating a plurality of constructs.

6. The method of claim 5 , further comprising:

determining the nucleic acid sequence of at least one of the plurality of constructs.

7. A nucleic acid construct comprising:

a lock region comprising a first primer binding region or a complement thereof;

a translation key region comprising a nucleic acid sequence that corresponds to an identity of a key or a complement thereof;

a message region comprising a nucleic acid sequence that corresponds to an encrypted message, wherein the encrypted message comprises an information format having each character that is converted into a triplet or quadruplet codon by employing the key, or a complement thereof,

a start region comprising a start codon, wherein the start region is in proximity to a 5′ end of the message region; and

a stop region comprising a stop codon, wherein the stop region is in proximity to a 3′ end of the message region,

wherein the key provides the identity of the start codon and the stop codon;

wherein the translation key region is disposed between the lock region and the message region.

8. The construct of claim 7 , wherein the message region comprises a buffered message region comprising a nucleic acid sequence that interrupts the information format.

9. The construct of claim 8 , further comprising:

a first leader region comprising a second primer binding region, wherein the leader region is in proximity to the 5′ end of the construct;

an optional second leader region comprising a third primer binding region, wherein the second leader region is disposed between the translation key region and the message region; and

a trailer region comprising a fourth primer binding region, wherein the trailer region is in proximity to the 3′ end of the construct.

10. The method of claim 1 , wherein each character is one of an alphanumeric character, a symbol, a punctuation mark, and a space.

11. The method of claim 1 , wherein the information format comprises a text, an image, or a matrix.

12. The method of claim 1 , wherein the key comprises a different codon corresponding to a different character, and wherein the different codon comprises three nucleotides.

13. The method of claim 1 , wherein the converting step comprises:

sectioning the information format into a plurality of sections; and

converting each character in each section with a different key, wherein each key comprises a different codon corresponding to a different character.

14. The method of claim 13 , wherein the translation key region comprises a nucleic acid sequence that corresponds to the identity of each of the different keys.

15. The method of claim 1 , wherein the converting step further comprises:

inserting one or more buffer regions in the encrypted message or a complement thereof, wherein each buffer region comprises a nucleic acid sequence that interrupts the information format, thereby generating a buffered, encrypted message represented by a plurality of nucleotides.

16. The method of claim 1 , wherein the lock region comprises of from about 15 to about 35 nucleotides.

17. The method of claim 1 , wherein the translation key region comprises of from about 15 to about 35 nucleotides.

18. The method of claim 1 , wherein the nucleic acid construct comprises a single-stranded sequence or a double-stranded sequence.

19. The construct of claim 7 , wherein the key comprises a different codon corresponding to a different character.

20. The construct of claim 7 , wherein each character is one of an alphanumeric character, a symbol, a punctuation mark, and a space.

Assignments (2)
CONFIRMATORY LICENSE Recorded Apr 20, 2023
From: NATIONAL TECHNOLOGY & ENGINEERING SOLUTIONS OF SANDIA, LLC
To: U.S. DEPARTMENT OF ENERGY
Reel/Frame 063407/0897 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 21, 2019
From: BACHAND, GEORGE; BACHAND, MARLENE; GOMEZ, ANDREW
To: NATIONAL TECHNOLOGY & ENGINEERING SOLUTIONS OF SANDIA, LLC
Reel/Frame 050121/0009 →
Continuity (1)
Provisional Application 62711964 · Jul 30, 2018
Cited By (3)
US 12,236,354 US 12,437,841 US 12,710,937