Integrated target waveguide devices and systems for optical coupling
Integrated target waveguide devices and optical analytical systems comprising such devices are provided. The target devices include an optical coupler that is optically coupled to an integrated waveguide and that is configured to receive optical input from an optical source through free space, particularly through a low numerical aperture interface. The devices and systems are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices provide for the efficient and reliable coupling of optical excitation energy from an optical source to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination. The devices and systems are well suited for miniaturization and high throughput.
1. An integrated target waveguide device comprising:
an optical coupler;
an integrated waveguide optically coupled to the optical coupler; and
a plurality of nanoscale sample wells optically coupled to the integrated waveguide;
wherein the optical coupler has a numerical aperture of no more than 0.10; and
wherein the optical coupler is at least 100 μm 2 in size.
2. The target waveguide device of claim 1 , wherein the optical coupler is a grating coupler.
3. The target waveguide device of claim 2 , wherein the grating coupler has blazed etching.
4. The target waveguide device of claim 2 , wherein the grating coupler has top-sided etching.
5. The target waveguide device of claim 2 , wherein the grating coupler has bottom-sided etching.
6. The target waveguide device of claim 2 , wherein the grating coupler has double-sided etching.
7. The target waveguide device of claim 2 , wherein the grating coupler has an overlay layer.
8. The target waveguide device of claim 7 , wherein the overlay layer is a silicon nitride layer or a silicon carbide layer.
9. The target waveguide device of claim 2 , wherein the grating coupler has double-sided etching and an overlay layer.
10. The target waveguide device of claim 2 , wherein the grating coupler is chirped.
11. The target waveguide device of claim 2 , wherein the grating coupler is a beam focusing coupler.
12. The target waveguide device of claim 11 , wherein the beam focusing coupler comprises a tapered waveguide region.
13. The target waveguide device of claim 11 , wherein the beam focusing coupler comprises a slab waveguide region.
14. The target waveguide device of claim 1 , further comprising a reflective layer positioned below the optical coupler.
15. The target waveguide device of claim 14 , wherein the reflective layer is a metallic layer.
16. The target waveguide device of claim 1 , further comprising a heat spreading layer in thermal contact with the optical coupler.
17. The target waveguide device of claim 16 , wherein the heat spreading layer is positioned directly below the optical coupler.
18. The target waveguide device of claim 16 , wherein the heat spreading layer comprises a heat conducting material.
19. The target waveguide device of claim 18 , wherein the heat conducting material is a metal.
20. The target waveguide device of claim 19 , wherein the metal is aluminum.
21. The target waveguide device of claim 16 , wherein the heat spreading layer is from 20 nm to 500 nm thick.
22. The target waveguide device of claim 21 , wherein the heat spreading layer is from 50 nm to 250 nm thick.
23. The target waveguide device of claim 1 , further comprising an alignment feature.
24. The target waveguide device of claim 23 , wherein the alignment feature comprises an alignment coupler.
25. The target waveguide device of claim 24 , wherein the alignment feature comprises a plurality of alignment couplers.
26. The target waveguide device of claim 23 , wherein the alignment feature comprises a reference mark.
27. The target waveguide device of claim 26 , wherein the reference mark comprises a fiducial or a patterned region.
28. The target waveguide device of claim 1 , comprising a plurality of optical couplers and a plurality of integrated waveguides optically coupled to the plurality of optical couplers.
29. The target waveguide device of claim 28 , wherein the device comprises at least four integrated waveguides optically coupled to at least four optical couplers.
30. The target waveguide device of claim 1 , wherein the device comprises at least 100 nanoscale sample wells optically coupled to the integrated waveguide.
31. The target waveguide device of claim 1 , wherein the plurality of nanoscale sample wells contain a DNA polymerase enzyme.