Methods and compositions for CNS delivery of iduronate-2-sulfatase
The present invention provides, among other things, compositions and methods for CNS delivery of lysosomal enzymes for effective treatment of lysosomal storage diseases. In some embodiments, the present invention includes a stable formulation for direct CNS intrathecal administration comprising an iduronate-2-sulfatase (I2S) protein, salt, and a polysorbate surfactant for the treatment of Hunters Syndrome.
1. A method of treating Hunter Syndrome comprising a step of administering intracerebroventricularly (ICV) to a human subject in need of treatment a formulation comprising an iduronate-2-sulfatase (I2S) protein at a concentration of at least 5 mg/ml at a dose amount of at least 10 mg.
2. The method of claim 1 , wherein the formulation contains a phosphate concentration no greater than 20 mM.
3. The method of claim 2 , wherein the formulation comprises phosphate at a concentration of no greater than 5 mM.
4. The method of claim 1 , wherein the I2S protein is present at a concentration of at least about 10 mg/ml.
5. The method of claim 1 , wherein the I2S protein is present at a concentration of approximately 30 mg/ml.
6. The method of claim 1 , wherein the I2S protein comprises an amino acid sequence of SEQ ID NO:1.
7. The method of claim 1 , wherein the formulation further comprises salt.
8. The method of claim 7 , wherein the salt is NaCl.
9. The method of claim 1 , wherein the formulation further comprises polysorbate.
10. The method of claim 9 , wherein the polysorbate is polysorbate 20 present at a concentration of approximately 0.005-0.02%.
11. The method of claim 1 , wherein the formulation has a pH of approximately 5.5-6.5.
12. The method of claim 1 , wherein the formulation is a liquid formulation.
13. The method of claim 1 , wherein the formulation is formulated as lyophilized dry powder.
14. The method of claim 1 , wherein the formulation further comprises NaCl at a concentration of approximately 154 mM, polysorbate 20 at a concentration of approximately 0.005%, and a pH of approximately 6.
15. The method of claim 1 , wherein the I2S protein is a synthetic, recombinant, gene-activated or natural enzyme.
16. The method of claim 1 , wherein the formulation is administered in a volume of about 1-15 ml.
17. A method of treating Hunter Syndrome, comprising a step of administering intracerebroventricularly (ICV) to a human subject in need of treatment a formulation comprising an iduronate-2-sulfatase (I2S) protein at a concentration at or greater than 5 mg/ml, salt at a concentration of approximately 154 mM, a polysorbate surfactant at a concentration of approximately 0-0.02%, and a pH of approximately 5.5-6.5; wherein the formulation is administered at a volume of about 1-5 ml, and wherein the formulation is administered at a dose amount of at least 10 mg.