Automated cell processing instruments comprising reagent cartridges
The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
1. An automated multi-module cell processing instrument comprising:
a housing;
a reagent cartridge, wherein the reagent cartridge comprises:
a thermal block with block reservoirs defined therein;
a cover disposed upon the thermal block with cover reservoirs defined therein, wherein the cover reservoirs and block reservoirs are coincident and together define reservoirs in the reagent cartridge;
an insert comprising a transformation module, wherein the transformation module is a flow-through electroporation device;
a script readable by a processor;
at least two additional inserts configured to reside within the reservoirs in the reagent cartridge; and
reagents dispensed in one or more of the reservoirs;
a growth module;
a cell concentration module;
an editing module; and
the processor.
2. The automated multi-module cell processing instrument of claim 1 , wherein the one or more reservoirs with reagents dispensed therein is sealed with a foil seal.
3. The automated multi-module cell processing instrument of claim 1 , further comprising a top cover disposed upon the cover wherein the top cover is configured to seal the reservoirs.
4. The automated multi-module cell processing instrument of claim 1 , wherein the growth module comprises a rotating growth vial with media and cells dispensed therein.
5. The automated multi-module cell processing instrument of claim 4 , wherein the cells are bacterial cells.
6. The automated multi-module cell processing instrument of claim 5 , wherein the cells are E. coli cells.
7. The automated multi-module cell processing instrument of claim 4 , wherein the cells are yeast cells.
8. The automated multi-module cell processing instrument of claim 7 , wherein the cells are S. cerevisiae cells.
9. The automated multi-module cell processing instrument of claim 4 , wherein the cells are plant cells.
10. The automated multi-module cell processing instrument of claim 4 , wherein the cells are animal cells.
11. The automated multi-module cell processing instrument of claim 10 , wherein the cells are mammalian cells.
12. The automated multi-module cell processing instrument of claim 1 , wherein one of the reagents dispensed in the one or more reservoirs comprises an antibiotic.
13. The automated multi-module cell processing instrument of claim 1 , wherein one of the reagents dispensed in the one or more reservoirs comprises a nucleic acid-guided nuclease.
14. The automated multi-module cell processing instrument of claim 13 , wherein the nucleic acid-guided nuclease is MAD7.
15. The automated multi-module cell processing instrument of claim 1 , wherein the editing module is configured to singulate cells.
16. An automated multi-module cell processing instrument comprising:
a housing;
a reagent cartridge for an automated multi-module cell processing instrument, wherein the reagent cartridge comprises:
a thermal block with block reservoirs defined therein;
a cover disposed upon the thermal block with cover reservoirs defined therein, wherein the cover reservoirs and block reservoirs are coincident and together define reservoirs in the reagent cartridge;
an insert comprising a transformation module;
a script readable by a processor;
at least two additional inserts configured to reside within the reservoirs in the reagent cartridge; and
reagents dispensed in one or more of the reservoirs;
a growth module;
a cell concentration module;
a singulation and editing module; and
the processor.
17. The automated multi-module cell processing instrument of claim 16 , wherein the singulation and editing module is a solid wall device.
18. The automated multi-module cell processing instrument of claim 16 , wherein the transformation module in the reagent cartridge is a flow-through electroporation device.
19. An automated multi-module cell processing instrument comprising:
a housing;
a reagent cartridge for an automated multi-module cell processing instrument, wherein the reagent cartridge comprises:
a thermal block with block reservoirs defined therein;
a cover disposed upon the thermal block with cover reservoirs defined therein, wherein the cover reservoirs and block reservoirs are coincident and together define reservoirs in the reagent cartridge;
an insert comprising a transformation module;
a script readable by a processor;
at least two additional inserts configured to reside within the reservoirs in the reagent cartridge; and
reagents dispensed in one or more of the reservoirs;
a growth module;
a cell concentration module; and
the processor.
20. The automated multi-module cell processing instrument of claim 19 , wherein the transformation module in the reagent cartridge comprises a flow-through electroporation device.