Treating patients harboring an isocitrate dehydrogenase-1 (IDH-1) mutation
Methods of treating patients diagnosed with AML or MDS harboring mutant IDH-1 include detecting an IDH1 mutation and the therapeutic administration of an inhibitor of a mutant IDH-1 as a single agent, or in combination with azacitidine (AZA) or cytarabine.
1. A method of treating acute myeloid leukemia (AML) in patients with an isocitrate dehydrogenase-1 (IDH1) mutation, the method comprising steps of:
a. isolating and purifying DNA from a sample obtained from a patient;
b. detecting an IDH1 mutation in the DNA from the sample; and
c. administering to the patient with the IDH1 mutation 150 mg of olutasidenib twice daily in a pharmaceutically acceptable composition.
2. The method of claim 1 , wherein the IDH1 mutation is an IDH1 R132 mutation.
3. The method of claim 2 , wherein the IDH1 R132 mutation is selected from the group consisting of R132C, R132H, R132S, R132G, and R132L.
4. The method of claim 1 , wherein the detecting an IDH1 mutation comprises detecting a single nucleotide variant (SNV) coding the IDH1 mutation, wherein the IDH1 mutation is selected from the group consisting of R132C, R132H, R132G, R132S, and R132L.
5. The method of claim 4 , wherein the IDH1 mutation is detected using polymerase chain reaction (PCR) technology with homogeneous real-time fluorescence detection.
6. The method of claim 1 , wherein the detecting an IDH1 mutation comprises using an in vitro PCR assay for the qualitative detection of single nucleotide variants (SNVs) coding an IDH1 R132 mutation selected from the group consisting of R132C, R132H, R132G, R132S, and R132L in the DNA from the sample.
7. The method of claim 1 , wherein the sample is obtained from patient bone marrow.
8. The method of claim 1 , wherein the sample is obtained from patient blood.
9. The method of claim 1 , wherein the pharmaceutically acceptable composition is formulated for oral administration.
10. The method of claim 9 , wherein the pharmaceutically acceptable composition is a capsule.
11. The method of claim 5 , wherein the method further comprises combining the extracted DNA sample with oligonucleotide primers designed to specifically amplify (i) R132C and R132H mutations or (ii) R132G, R132S, and R132L mutations.
12. The method of claim 11 , wherein the real-time fluorescence signal of each IDH1 mutation of either (i) R132C and R132H or (ii) R132G, R132S, and R132L is distinguishable in a single well.
13. A method of treating acute myeloid leukemia (AML) in patients with an isocitrate dehydrogenase-1 (IDH1) mutation, the method comprising administering twice daily to a patient with an IDH1 mutation 150 mg of olutasidenib in a pharmaceutically acceptable composition, wherein the IDH1 mutation has been detected using an FDA-approved diagnostic test.
14. The method of claim 13 , wherein the FDA-approved diagnostic test comprises detecting an IDH1 mutation in DNA from a sample obtained from the patient.
15. The method of claim 13 , wherein the patient is receiving or has received therapy comprising azacitidine or cytarabine.
16. The method of claim 13 , wherein the AML, is relapsed or refractory.
17. A method of treating AML in patients with an isocitrate dehydrogenase-1 (IDH1) mutation, the method comprising steps of:
determining whether the patient has an IDH1 mutation by:
i. obtaining a sample from the patient; and
ii. performing an assay on the sample to determine if the patient has an IDH1 mutation; and
if the patient has an IDH1 mutation, then administering to the patient with the IDH1 mutation 150 mg of olutasidenib twice daily in a pharmaceutically acceptable composition, and
if the patient does not have an IDH1 mutation, then not administering to the patient with the IDH1 mutation 150 mg of olutasidenib twice daily in a pharmaceutically acceptable composition.
18. The method of claim 17 , wherein the IDH1 mutation is an IDH1 R132 mutation.
19. The method of claim 18 , wherein the IDH1 R132 mutation is selected from the group consisting of R132C, R132H, R132S, R132G, and R132L.
20. The method of claim 17 , wherein the assay comprises detecting an IDH1 mutation in DNA from a sample obtained from the patient.
21. A method of treating an adult patient with acute myeloid leukemia (AML) with a susceptible IDH1 mutation as detected by an FDA-approved test, the method comprising administering to the patient in need thereof 150 mg of olutasidenib twice daily in a pharmaceutically acceptable composition.
22. The method of claim 21 , wherein the AML is relapsed or refractory acute myeloid leukemia.
23. The method of claim 22 , further comprising selecting the patient for the treatment of AML prior to the administration of olutasidenib based on the presence of an IDH1 mutation in the blood or bone marrow.
24. The method of claim 22 , wherein the patient is identified as harboring an IDH1 mutation selected from the group consisting of R132C, R132H, R132S, R132G, and R132L.