Compositions of beta-glucuronidase enzyme blends with enhanced enzymatic activity and methods of preparation thereof
Blends of enzymes of the same enzyme class (same EC number), such as beta-glucuronidase enzyme blends, are provided that exhibit synergistic levels of activity across a range of substrates as compared to the activity levels of each enzyme in the blend individually. The blends also may exhibit a greater effective substrate range, as well as greater effective pH and/or temperature ranges as compared to single enzyme preparations. Predictive methods for determining optimal enzyme blends, methods for preparing enzyme blends, enzyme blend compositions, enzyme blend formulations and methods of using such enzyme blends are provided.
1. A composition comprising a blend of Brachyspira pilosicoli beta-glucuronidase (BpGUS) and Eubacterium eligens beta-glucuronidase (EeGUS), wherein:
(a) the blend comprises about 10%-30% BpGUS and about 70%-90% EeGUS; or
(b) the blend comprises about 15%-25% BpGUS and about 75%-85% EeGUS; or
(c) the blend comprises 15% BpGUS and 85% EeGUS; or
(d) the blend comprises 25% BpGUS and 75% EeGUS; and
wherein BpGUS comprises the amino acid sequence shown in SEQ ID NO: 5 and EeGUS comprises the amino acid sequence shown in SEQ ID NO: 10.
2. A formulation comprising the composition of claim 1 , wherein the formulation comprises the enzyme blend and at least one excipient, wherein each enzyme in the blend is present at a concentration of at least 0.1 mg/mL.
3. The formulation of claim 2 , wherein:
(a) the formulation is an aqueous formulation;
(b) the formulation is a lyophilized formulation;
(c) the at least one excipient is selected from the group consisting of water, salts, buffers, sugars and amino acids; and/or
(d) the formulation is free of polymers and detergents.
4. A packaged formulation comprising the formulation of claim 2 and a container.
5. The composition of claim 1 , wherein the blend comprises about 10%-30% BpGUS and about 70%-90% EeGUS.
6. The composition of claim 1 , wherein the blend comprises about 15%-25% BpGUS and about 75%-85% EeGUS.
7. The composition of claim 1 , wherein the blend comprises 15% BpGUS and 85% EeGUS.
8. The composition of claim 1 , wherein the blend comprises 25% BpGUS and 75% EeGUS.