Compositions, methods and uses for improved human papilloma virus constructs
View Patent ↗Embodiments of the present invention provide compositions and methods for recombinantly generating tagless constructs of proteins or peptides. In certain embodiments, recombinant proteins or peptides disclosed herein concern human papilloma virus (HPV). Other embodiments concern using these constructs in compositions to elicit immune responses in a subject to one or more HPV types. Therapeutic and prophylactic vaccines for the prevention and treatment of viral infections are also disclosed. Nucleic acids and expression vectors coding for constructs contemplated herein are provided. In certain embodiments, an HPV capsid protein generated is devoid of any fusion tags. In addition, truncated forms of HPV L1 are contemplated.
1. A bacterial plasmid construct comprising:
a target Human Papilloma Virus (HPV) DNA molecule comprising a transcription start codon, wherein the target HPV DNA molecule encodes one or more proteins or peptides having at least 3 amino acids and no more than 24 amino acids deleted at the N-terminus, and up to 29 amino acids deleted at the C-terminus, the HPV DNA molecule comprising one or more of:
1) the nucleic acid represented by SEQ ID NO: 1;
2) the nucleic acid represented by SEQ ID NO: 2;
3) the nucleic acid represented by SEQ ID NO: 3;
4) the nucleic acid represented by SEQ ID NO: 4;
5) the nucleic acid represented by SEQ ID NO: 5;
6) the nucleic acid represented by SEQ ID NO: 6;
7) the nucleic acid represented by SEQ ID NO: 7; or
8) the nucleic acid represented by SEQ ID NO: 8;
a promoter associated with the target HPV DNA molecule;
a restriction site positioned between the promoter and the target HPV DNA molecule;
a resistance gene capable of conferring resistance to a selection agent; and
the plasmid construct is devoid of any tag, or tracking agent, making the construct tagless.
2. The bacterial plasmid construct of claim 1 , wherein the target HPV DNA molecule encodes a single protein or a chimeric protein.
3. The bacterial plasmid construct of claim 1 , wherein the target HPV DNA molecule encodes one or more proteins or peptides of use in an immunogenic composition against a target HPV.
4. The bacterial plasmid construct of claim 1 , wherein the encoded molecule forms part of a capsomere.
5. The bacterial plasmid construct of claim 1 , wherein the promoter is a Tac promoter.
6. A pharmaceutical composition comprising one or more proteins or peptides expressed from one or more bacterial constructs according to claim 1 and a pharmaceutically acceptable carrier.
7. The pharmaceutical composition according to claim 6 , wherein the pharmaceutical composition is part of an immunogenic composition against one or more HPV type.
8. The pharmaceutical composition according to claim 7 , wherein the pharmaceutical composition is part of an immunogenic composition against one or more HPV type selected from the group consisting of HPV16, HPV18, HPV31, HPV33, HPV35, HPV45, HPV52 and HPV58.
9. A method for eliciting an immune response to at least one HPV type in a subject, the method comprising administering to the subject a composition comprising the pharmaceutical composition of claim 6 and eliciting an immune response in the subject to the at least one HPV type.
10. A prophylactic method of treatment for eliciting an immune response to at least one HPV type in a subject comprising, administering to the subject, a prophylactically effective amount of a composition comprising the pharmaceutical composition of claim 6 and reducing or preventing infection to the at least one HPV type in the subject.
11. A therapeutic treatment method for eliciting an immune response to at least one HPV type infection in a subject comprising, administering to the subject a therapeutically effective amount of a pharmaceutical composition according to claim 6 and treating the HPV infection in the subject.
12. An isolated cell comprising the bacterial plasmid construct of claim 1 .
13. An isolated preparation of expressed inclusion bodies comprising a polypeptide encoded by the bacterial plasmid construct of claim 1 .
14. The bacterial plasmid construct of claim 1 , wherein the plasmid bacterial construct is void of a GST tag.
15. The bacterial plasmid construct of claim 1 , wherein the encoded proteins or peptides form a pentamer.
16. The bacterial plasmid construct of claim 1 , wherein the bacterial plasmid construct does not encode a fusion protein or fusion peptide.
17. A kit comprising at least one of a bacterial plasmid construct and an encoded protein or peptide of the bacterial plasmid construct of claim 1 and at least one container.
18. The kit of claim 17 , wherein the at least one of a bacterial construct and an encoded protein or peptide of the bacterial plasmid construct is in the form of a pharmaceutically acceptable composition further comprising a pharmaceutically acceptable carrier.
19. The bacterial plasmid construct of claim 1 , wherein the target HPV DNA molecules encodes a truncated HPV protein or peptide of 3, 5, 9, 13, 15, 16, or 20 amino acids deleted at the N-terminus of the encoded protein or peptide.
20. The bacterial plasmid construct of claim 1 , wherein the HPV DNA molecule encodes a truncated HPV protein or peptide with 3 amino acids deleted at the N-terminus and up to 29 amino acids deleted at the C-terminus.
21. The bacterial plasmid construct of claim 1 , wherein the HPV DNA molecule encodes a truncated HPV protein or peptide with 29 amino acids deleted at the C-terminus.