Protection against recurrent genital herpes by therapeutic immunization with herpes simplex virus type 2 ribonucleotide reductase protein subunits
The present invention relates to compositions comprising HSV antigens and methods for their use in the treatment or prevention of asymptomatic and symptomatic herpesvirus infection, or recurrence.
1. A method of treating a herpes simplex type I (HSV1) or herpes simplex virus 2 (HSV2) related infection in a subject, the method comprising contacting a cell of the subject with a therapeutically effective amount of a non-viral composition selected from the group consisting of:
a) a composition comprising a combination of at least two HSV antigens selected from the group consisting of RR1, RR2, VP22, UL25, VP16, VP13/14, and VP11/12; and
b) a composition comprising at least one recombinant nucleic acid molecule encoding a combination of-at least two HSV antigens selected from the group consisting of RR1, RR2, VP22, UL25, VP16, VP13/14, and VP11/12.
2. The method of claim 1 , wherein the composition is administered as a prime vaccine in a prime and pull treatment regimen.
3. The method of claim 2 , wherein the pull treatment of the prime and pull treatment regimen comprises administration of at least one cytokine selected from the group consisting of CXCL9, CXCL10 and CXCL11.
4. The method of claim 1 , wherein the herpesvirus-associated disorder is selected from the group consisting of labial herpes, genital herpes, primary herpes infection with a human alpha-herpesvirus, Bell's palsy, vestibular neuritis, and herpetic neuralgia.
5. The method of claim 1 , wherein the subject has an asymptomatic herpesvirus infection.
6. The method of claim 1 , wherein the composition comprises a combination of an HSV RR2 antigen and at least one additional HSV antigen.
7. The method of claim 6 , wherein the at least one additional HSV antigen is selected from the group consisting of RR1, VP22, UL25, VP16, VP13/14, and VP11/12.
8. The method of claim 1 , wherein the composition comprises at least one recombinant nucleic acid molecule encoding a combination of an HSV RR2 antigen at least one additional HSV antigen.
9. The method of claim 8 , wherein the at least one additional HSV antigen is selected from the group consisting of RR1, VP22, UL25, VP16, VP13/14, and VP11/12.
10. The method of claim 1 , wherein the composition further comprises at least one additional HSV antigen.
11. The method of claim 10 , wherein the at least one additional HSV antigen is selected from the group consisting of gD, RR1, RR2, VP22, gB, VP16, VP13/14, VP11/12 and UL25.
12. The method of claim 10 , wherein the at least one additional HSV antigen is selected from the group consisting of gD and gB.