Composition comprising nucleic acids of parasitic, pathogenic or infesting biological systems for inhibiting and/or controlling the growth of said systems and process for the preparation thereof
The present disclosure describes a DNA fragment mixture for the prevention or for the treatment of at least one pathogenic, parasitic or infesting species of plants or of the environment, wherein the DNA mixture consists of random fragments of total DNA of at least one pathogenic, parasitic, or infesting species, and/or at least one phylogenetically similar species, against which the prevention and treatment are directed. Further, the disclosure describes a process and related system for improvement of the production/growth of microorganisms at high yield in bioreactors or photobioreactors, or of plants in different culture systems, where the nucleic acids of the organisms produced/grown by such a process are removed from the culture medium and the culture medium, deprived of these nucleic acid, is used again in the process.
1. A method for growing an organism in a culture system comprising a culture medium, the method comprising
removing from the culture medium extracellular DNA of the organism to provide a regenerated medium; and
culturing the organism in the regenerated medium,
wherein the organism is a microorganism or a plant.
2. The method according to claim 1 , wherein the culture medium is within a bioreactor, a photobioreactor or a system for hydroponic culture.
3. The method according to claim 2 , wherein the removing is performed by an external removal unit external to the bioreactor, photobioreactor or system for hydroponic culture.
4. The method according to claim 3 , wherein the external removal unit comprises an external container and a system for generating of electric, or magnetic, or electromagnetic fields and displacing DNA from the bioreactor, the photobioreactor or a system for hydroponic culture.
5. The method according to claim 2 , wherein the removing is performed by an internal removal unit integrated into the bioreactor, photobioreactor or system for hydroponic culture.
6. The method according to claim 5 , wherein the internal removal unit comprises a system for generating electric, or magnetic, or electromagnetic fields and displacing DNA from the culture medium, the system integrated within the bioreactor, photobioreactor or system for hydroponic culture.
7. The method according to claim 1 , wherein the removing is performed by applying to the culture medium an electric, magnetic, or electromagnetic field to remove the extracellular DNA from the culture medium.
8. The method according to claim 1 , wherein the removing is performed by centrifugating the culture medium, to remove the extracellular DNA from the culture medium.
9. The method according to claim 1 , wherein the removing is performed by filtrating the culture medium to remove the extracellular DNA from the culture medium.
10. The method according to claim 1 , wherein the removing is performed by treating the culture medium with degrading enzymes, to remove the extracellular DNA from the culture medium.
11. The method according to claim 1 , wherein the removing is performed by heat treating the culture medium to remove the extracellular DNA from the culture medium.
12. The method according to claim 1 , wherein the removing is performed by performing acidifying treatments of the culture medium to remove the extracellular DNA from the culture medium.
13. The method according to claim 1 , further comprising collecting the extracellular DNA removed from the culture medium.
14. The method according to claim 1 , wherein the DNA comprises fragments of 80 to 800 bp.