Brassica plant comprising a mutant indehiscent allele
This invention relates to crop plants of which the fruit dehiscence properties are modulated. More specifically the invention relates to improved methods and means for reducing seed shattering, or delaying seed shattering until after harvest, in plants, while maintaining at the same time an agronomically relevant threshability of the pods.
1. A Brassica plant comprising at least two IND genes, or a cell, part, seed or progeny thereof, comprising only two full knock-out mutant IND alleles in its genome, wherein said full knock-out mutant IND alleles are mutant alleles of a gene encoding a functional INDEHISCENT (IND) protein comprising a nucleic acid that:
(a) has at least 95% sequence identity to the nucleic acid sequence of SEQ ID NO: 1 or SEQ ID NO: 5; or
(b) encodes a protein having an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2,
wherein said full knock-out mutant IND alleles comprise a mutated DNA region comprising one or more inserted, deleted, or substituted nucleotides compared to a corresponding wild-type DNA region in the functional IND gene, and wherein said full knock-out mutants IND allele are:
(i) a mutant IND allele comprising a mutation resulting in the production of a non-functional IND protein or resulting in no production of an IND protein,
(ii) a mutant IND allele encoding a truncated IND protein of which at least the Helix-Loop-Helix domain at positions corresponding to positions 132 to 174 of SEQ ID NO: 10 is lacking or substituted; and/or
(iii) a mutant IND allele with a missense mutation of the codon encoding the amino acid at a position corresponding to position 128 of SEQ ID NO: 10, and wherein the plant has increased seed yield compared to the seed yield of a corresponding plant not comprising full knock-out mutant IND alleles.
2. The Brassica plant according to claim 1 , wherein said full knock-out mutant alleles have the sequence of SEQ ID NO: 1 in which the c at position 364 is substituted with t, or in which the g at position 307 is substituted with a and the g at position 380 is substituted with a, wherein said plant has increased seed yield compared to the seed yield of a corresponding plant not comprising full knock-out mutant IND alleles.
3. The Brassica plant of claim 1 , which is a Brassica napus, Brassica juncea , or Brassica carinata plant.
4. The Brassica plant of claim 2 , reference seed comprising said allele having the sequence of SEQ ID NO: 1 in which the c at position 364 is substituted with t having been deposited at the ATCC, under accession number PTA-8796, and reference seed comprising said allele having the sequence of SEQ ID NO: 1 in which the g at position 307 is substituted with a and the g at position 380 is substituted with a, having been deposited at the ATCC, under accession number PTA-8795.
5. The Brassica plant of claim 1 , wherein said two full knock-out mutant IND alleles are homozygous.
6. A seed pod obtainable from the plant of claim 1 , wherein said seed pod comprises said two full knock-out mutant IND alleles.
7. A cell, part, seed or progeny of the plant of claim 1 , said cell, part, seed or progeny comprising said two full knock-out mutant IND alleles.
8. A method for making a plant according to claim 1 , wherein the method comprises:
(a) generating and/or identifying two or more plants each comprising one or more of said mutant IND alleles,
(b) crossing a first plant comprising one or more of said mutant IND alleles with a second plant comprising one or more said mutant IND alleles, collecting F1 seeds from the cross, and, identifying an F1 plant comprising one or more of said mutant IND alleles from the first plant with one or more of said mutant IND alleles from the second plant, and
(c) optionally, repeating step (b) until an F1 plant comprising said two full knock-out mutant IND alleles is obtained.
9. A method for making a hybrid Brassica seed or plant according to claim 1 comprising crossing a first plant comprising two full knock-out mutant IND alleles as described in claim 1 in homozygous state and a second plant comprising two full knock-out mutant IND alleles as described in claim 1 in homozygous state, and collecting F1 hybrid seeds from the cross.
10. The method of claim 9 , wherein the full knock-out mutant IND alleles in the first and the second plant are the same.
11. The method according to claim 9 , wherein the first plant is used as a male parent plant and the second plant is used as a female parent plant.