IL-10 EGFR antibody fusion protein and uses thereof
The present disclosure provides proteinaceous complexes, pharmaceutical compositions, medicaments and/or kits comprising the proteinaceous complexes, methods for producing the proteinaceous complexes, and uses thereof.
1. A proteinaceous complex formed by:
a light chain and a heavy chain of an antibody specific for EGFR;
wherein the variable domain of said light chain and the variable domain of said heavy chain respectively comprise SEQ ID NOs: 37 and 38; and
(ii) a fusion polypeptide comprising, from N-terminus to C-terminus, a cytokine fused to an antibody Fc region via a linker, wherein the cytokine is interleukin 10;
wherein the amino acid sequence of the fusion polypeptide comprises SEQ ID NO: 51; and
wherein said antibody Fc region of the fusion polypeptide of (ii) complexes with the antibody heavy chain of (i) to form a dimer.
2. A pharmaceutical composition comprising the proteinaceous complex of claim 1 and a pharmaceutically acceptable carrier.
3. A method for inhibiting growth of a tumor or a tumor cell, comprising contacting said tumor or tumor cell with an effective amount of the proteinaceous complex of claim 1 .
4. A method for treating cancer in a patient, comprising administering a therapeutically effective amount of the proteinaceous complex of claim 1 .