Systems and methods for inspecting a microfluidic rotor device
Described herein are various embodiments directed to rotor devices, systems, and kits. Embodiments of rotors disclosed herein may be used to characterize one or more analytes of a fluid. A method may include aligning an apparatus to an imaging device. The apparatus may include a set of wells defined by a first layer coupled to a second layer. The first layer may be substantially transparent to infrared radiation. The second layer may define a channel. The second layer may be substantially absorbent to the infrared radiation. The apparatus may further include a third layer coupled to the second layer and define an opening configured to receive a fluid. The third layer may be substantially transparent to the infrared radiation. A set of images of the apparatus may be generated using the imaging device. Bonding information may be generated based on the set of images.
1. A method for inspecting a microfluidic rotor, comprising:
aligning a microfluidic rotor to an imaging device, the microfluidic rotor including a set of wells defined by a first layer coupled to a second layer, the first layer being substantially transparent to infrared radiation, the second layer defining a channel, the second layer being substantially absorbent to the infrared radiation, the microfluidic rotor further including a third layer coupled to the second layer and defining an opening configured to receive a fluid, the third layer being substantially transparent to the infrared radiation;
generating a set of images of at least a portion of the microfluidic rotor using the imaging device;
generating bonding information based on the set of images, the bonding information including a set of edges and gaps formed between the second layer and the third layer; and
classifying a weld quality of the microfluidic rotor using the bonding information.
2. The method of claim 1 , wherein the set of images includes one or more of a plan view of the microfluidic rotor, a bottom view of the microfluidic rotor, a side view, and a skew view of the microfluidic rotor.
3. The method of claim 1 , the generating the set of images further includes illuminating the microfluidic rotor.
4. The method of claim 3 , the illuminating the microfluidic rotor includes employing diffuse axial illumination.
5. The method of claim 1 , the classifying the weld quality further includes identifying one or more of a number, size, shape, and location of a set of defects in the microfluidic rotor.
6. The method of claim 5 , the classifying the microfluidic rotor includes a set of rotor classifications including one or more of rejected, acceptable, limited release, and requiring secondary inspection.
7. The method of claim 1 , the aligning the microfluidic rotor includes orienting the imaging device parallel to the microfluidic rotor.
8. The method of claim 1 , the aligning the microfluidic rotor includes orienting the imaging device perpendicular to the microfluidic rotor.
9. A method for inspecting a microfluidic rotor, comprising:
aligning a microfluidic rotor to an imaging device, the microfluidic rotor including a set of wells defined by a first layer coupled to a second layer, the first layer being substantially transparent to infrared radiation, and the second layer defining a channel, the second layer being substantially absorbent to the infrared radiation, and the microfluidic rotor further including a third layer coupled to the second layer and defining an opening configured to receive a fluid, the third layer being substantially transparent to the infrared radiation, wherein one or more wells of the set of wells includes a reagent;
generating a set of reagent images using the imaging device;
generating reagent information from the reagent images, the reagent information including a shape and size of the reagent; and
classifying a reagent quality using the reagent information.
10. The method of claim 9 , wherein the set of reagent images includes one or more of a plan view of the reagent, a bottom view of the reagent, and a side view of the reagent.
11. The method of claim 9 , further comprising illuminating the reagent when generating the reagent images.
12. The method of claim 11 , the illuminating the reagent includes employing diffuse axial illumination.
13. The method of claim 9 , the classifying the reagent quality includes identifying one or more of a number, size, shape, color, and location of the reagent in the apparatus.
14. The method of claim 13 , the classifying the reagent quality includes a set of rotor classifications including one or more of rejected, acceptable, limited release, and requiring secondary inspection.
15. The method of claim 9 , the aligning the apparatus includes orienting the imaging device parallel to the apparatus.
16. The method of claim 9 , the aligning the apparatus includes orienting the imaging device perpendicular to the apparatus.
17. The method of claim 9 , wherein the reagent is a lyophilized reagent.