Decellularization of plant cell culture materials for tissue engineering and drug delivery
Provided herein are enzymatically decellularized cells, and methods of producing said cells, that can be used in a scaffold. The scaffolds featured herein are biocompatible and can comprise decellularized cells that have been modified to express a bioactive agent or molecule.
1. A method for decellularizing cells, the method comprising:
contacting a plurality of plant cells with a composition comprising a nuclease comprising DNase, thereby decellularizing the plurality of plant cells, wherein the plurality of plant cells are cellulose producing plant cells.
2. The method of claim 1 , wherein the DNase is DNaseI.
3. The method of claim 1 further comprising culturing the plurality of plant cells prior to contacting the cells with the nuclease.
4. The method of claim 3 , further comprising isolating cellular material from the cultured plurality of plant cells.
5. A method of producing a biocompatible scaffold in vitro, the method comprising:
contacting cellulose producing cultured plant cells with a composition comprising a DNase, thereby producing decellularized cultured plant cells; and
creating a biocompatible scaffold in vitro from the decellularized [modified] cultured plant cells.
6. The method of claim 5 further comprising modifying the cellulose producing cultured plant cells to express a bioactive agent.
7. The method of claim 6 , wherein the bioactive agent is a molecule that directs mammalian cell expansion, differentiation, or a cellular response.
8. The method of claim 6 , wherein the bioactive agent is imbedded, attached, or associated with cell walls of the cellulose producing cultured plant cells.
9. The method of claim 5 , wherein the DNase is DNaseI.
10. The method of claim 5 , wherein the composition further comprises trypsin, collagenase, lipase, dispase, thermolysin, and α-galactosidase.
11. The method of claim 1 further comprising, prior to contacting, subjecting the plurality of cultured plant cells to lyophilization.
12. The method of claim 5 further comprising, prior to contacting, subjecting the cultured plant cells to lyophilization.