Method for measuring fibroblast growth factor-23 and reagent therefor
A method for measuring fibroblast growth factor-23 (FGF-23) in a sample, which comprise the following steps: (1) reacting, in an aqueous medium, FGF-23 in a sample with magnetic particles, a first antibody or a fragment thereof which binds to FGF-23, and a second antibody or a fragment thereof which binds to FGF-23, to form on the magnetic particles an immunocomplex comprising the first antibody or a fragment thereof which binds to FGF-23. FGF-23, and the second antibody or a fragment thereof which binds to FGF-23; (2) collecting the magnetic particles in the reaction mixture after step (1) by magnetic force, and separating the magnetic particles collected by magnetic force from the other components; and (3) measuring the immunocomplex on the magnetic particles separated in step (2). The present invention provides a method for measuring FGF-23 in a sample, which have a high sensitivity and have a wide measurement range.
1. A method for measuring fibroblast growth factor-23 (FGF-23) in a sample, wherein the method comprises the steps of:
(1) reacting, in an aqueous medium, FGF-23 in a sample with a magnetic particle bonded with a first antibody or a fragment thereof which binds to FGF-23, and a second antibody or a fragment thereof which binds to FGF-23, to form on the magnetic particle an immunocomplex comprising the first antibody or a fragment thereof which binds to FGF-23, FGF-23, and the second antibody or a fragment thereof which binds to FGF-23,
wherein the sample comprises FGF-23 in a concentration ranging from 5 pg/mL to 10,000 pg/mL;
(2) collecting the magnetic particle in the reaction mixture after step (1) by magnetic force, and separating the magnetic particle collected by magnetic force from the other components; and
(3) measuring the immunocomplex on the magnetic particle separated in step (2).
2. The method of claim 1 , wherein the second antibody is a labeled antibody.
3. The method of claim 2 , wherein the labeled antibody is an alkaline phosphatase-labeled antibody.
4. The method of claim 1 , wherein measurement of the immunocomplex on the magnetic particle in step (3) is carried out by measurement of chemiluminescence.
5. The method of claim 1 , wherein the sample is serum or plasma.
6. The method of claim 1 , wherein the sample comprises FGF-23 in a concentration ranging from 5 pg/mL to 3,000 pg/mL.
7. The method of claim 1 , wherein the sample comprises FGF-23 in a concentration ranging from 5 pg/mL to 1,000 pg/mL.