Bioreactor paradigm for the production of secondary extra-particle hyphal matrices
The invention describes a methodology for production of a secondary extra-particle fungal matrix for application as a mycological material, manufactured via a Type II actively aerated static packed-bed bioreactor. A pre-conditioned air stream is passed through a substrate of discrete elements inoculated with a filamentous fungus to form an isotropic inter-particle hyphal matrix between the discrete elements. Continued feeding of the air through the substrate of discrete elements and isotropic inter-particle hyphal matrixes develops an extra-particle hyphal matrix that extends from an isotropic inter-particle hyphal matrix in the direction of airflow into a void space within the vessel.
1. A method of producing a mycological material comprising the steps of
providing a vessel having a chamber;
loading a substrate of discrete elements inoculated with a filamentous fungus into said chamber;
feeding a pre-conditioned air stream through said vessel for diffusion between said discrete elements in said chamber and for a time sufficient for said filamentous fungus to expand a contiguous network of hyphae between and around said discrete elements to form an isotropic inter-particle hyphal matrix; and
continuing to feed said pre-conditioned air stream through said vessel for diffusion between said discrete elements and said isotropic inter-particle hyphal matrix for a time sufficient to develop a polarized condition within said vessel wherein air exits said isotropic inter-particle hyphal matrix as a laminar flow into at least one void space within said vessel and to form an extra-particle hyphal matrix extending from said isotropic inter-particle hyphal matrix in the direction of airflow within said at least void space.
2. A method as set forth in claim 1 wherein said vessel has a permeable partition within said vessel separating said chamber from said void space and said air stream is fed through said vessel passing downwardly through said chamber into said void space.
3. A method as set forth in claim 1 wherein said air stream is fed through said vessel passing upwardly through said chamber into said void space.
4. A method as set forth in claim 1 wherein said step of loading includes separating said substrate of discrete elements inoculated with a filamentous fungus into two spaced apart sections within said chamber.
5. A method as set forth in claim 4 wherein said pre-conditioned air stream is fed through said vessel for diffusion between said discrete elements in each said section to form an isotropic inter-particle hyphal matrix therein and to form an extra-particle hyphal matrix extending from said isotropic inter-particle hyphal matrix in the direction of airflow.
6. A method as set forth in claim 1 wherein said vessel is disposed vertically and said air stream flows vertically through each said section.
7. A method as set forth in claim 1 wherein said vessel is disposed horizontally and said air stream flows horizontally through each said section.
8. A method as set forth in claim 1 further comprising the step of introducing at least one of a paramorphogen, a volatile compound and an aromatic compound into said pre-conditioned air stream prior to diffusion between said discrete elements to modify the specific morphology and density of said extra-particle hyphal matrix.
9. A method as set forth in claim 1 further comprising the steps positioning a a second low-density substrate in said at least one void space within said vessel and allowing said extra-particle hyphal matrix to grow around and within said second low-density substrate to form a composite of said extra-particle hyphal matrix and said second low-density substrate.
10. A method as set forth in claim 9 wherein said at least one void space has a selected geometric shape and said composite forms into said shape.
11. A method as set forth in claim 1 wherein said at least one void space has a selected geometric shape and said extra-particle hyphal matrix forms into said shape.