DNA vector and transformed tumor cell vaccines
Customized whole cell cancer vaccines can be produced from autologous (ex vivo or in situ) or allogeneic human or veterinary patient cell lines. Cells are transformed with S. pyogenes DNA that expresses an Emm protein on the cell surface and cytosol. Treatment of cancer patients with an Emm vector vaccine induces an immunologic response to the cancer by enhancing immunogenicity of a tumor. Emm vaccines can be used in patients where the cancer is not identified due to lower tumor burden or used to treat a specific cancer and subsequently treat for a second type that may have arisen through metastasis.
1. A method for preparing a membrane anchoring protein, comprising:
inserting a first DNA encoding the amino acid sequence of SEQ ID NO: 7 into the N-terminal signal region of a DNA encoding an M serotype 55 group A streptococcus protein;
inserting a second DNA encoding the amino acid sequence of SEQ ID NO: 9 into the C-terminal anchor region of the DNA encoding said protein;
wherein the encoded protein expressed from the modified DNA is a membrane anchoring protein.
2. The method of claim 1 wherein the M serotype 55 group A streptococcus protein is a gram positive bacterial cell wall protein.
3. The method of claim 2 wherein the gram positive bacterial wall protein is from a serotype of group A Streptococcus.
4. The method of claim 1 wherein the encoded protein has the amino acid sequence of SEQ ID NO: 5.
5. The method of claim 1 wherein the membrane anchoring protein anchors to a cell membrane.
6. The method of claim 5 wherein the cell membrane is a eukaryotic membrane.
7. The method of claim 5 wherein the cell membrane is a prokaryotic membrane.