Method for sample separation and collection
A centrifuge device and method for use are presented. The centrifuge device includes a housing, a chamber, a channel, and a cover. The housing includes a first port and a vent opening and is designed to rotate about an axis passing through a center of the housing. The chamber is defined within the housing and is coupled to the first port. A first portion of the chamber has a width that tapers between a first width at a first position and a second width at a second position within the chamber, the first width being greater than the second width. The channel is coupled to the second position of the chamber and arranged such that a path exists for gas to travel from the channel to the vent opening. The cover provides a wall that seals the chamber.
1. A method, comprising:
placing a sample into a centrifuge chamber via a first port, wherein the centrifuge chamber is defined within a cylindrical housing;
sealing the first port to prevent leakage of the sample back through the first port;
rotating the centrifuge chamber about an axis passing through a center of the cylindrical housing;
separating the sample within the chamber due to the rotation, wherein a first portion of the sample moves into a first portion of the chamber that extends along a circumference of the cylindrical housing, the first portion of the chamber having a tapering width as it extends along the circumference of the cylindrical housing, and a second portion of the sample moves into a second portion of the chamber that extends radially from the axis passing through the center of the cylindrical housing;
venting gas within the centrifuge chamber through a vent channel coupled to the first portion of the chamber to a vent opening; and
extracting the second portion of the sample via a second port.
2. The method of claim 1 , wherein the second port is positioned substantially above the second portion of the chamber.
3. The method of claim 1 , wherein the rotating comprises rotating the centrifuge chamber between 5,000 and 20,000 RPM.
4. The method of claim 1 , wherein the extracting comprises extracting the second portion of the sample via an applied pressure differential at the second port.
5. The method of claim 1 , wherein the first portion of the sample has a higher density than the second portion of the sample.
6. The method of claim 1 , further comprising stopping the rotation of the chamber before the extracting.