IP Library Granted Patent US 12,570,992
Granted Patent B2
US 12,570,992 · App. 16/554,345 · Granted Mar 10, 2026

Targeted modification of maize roots to enhance abiotic stress tolerance

Inventors: Shawn Michael Kaeppler (Oregon, WI); Patompong Saengwilai (Bangkok, TH); Jonathan Paul Lynch (Boalsburg, PA); Malcolm John Bennett (Nottingham, GB); James Johnson (Whitestown, IN)
Assignees: Wisconsin Alumni Research Foundation; The Penn State Research Foundation; The University of Nottingham
C12N15/8271A01H1/06A01H5/10A01H6/4684C07K14/415C12N15/82C12N15/8242
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,570,992
App. No.
16/554,345
Granted
Mar 10, 2026
Kind
B2
Abstract

The present invention relates to crop breeding. More particularly, the present invention relates to targeted modification of root to enhance abiotic stress tolerance in maize. In one aspect, the invention provides recombinant maize exhibiting increased root cortical aerenchyma (RCA). Methods of making the recombinant maize and various methods of plant selection and breeding are further provided.

Claims (5)

1 . A recombinant maize plant, comprising a construct,

wherein the construct comprises a polynucleotide sequence encoding the sense strand and the antisense strand of all of SEQ ID NO: 1 operably linked to a heterologous promoter such that a function of a protein encoded by SEQ ID NO: 1 is decreased in the recombinant maize plant, wherein the recombinant maize plant exhibits decreased root cortical aerenchyma (RCA) as compared to a maize plant lacking the construct.

2 . The recombinant maize plant of claim 1 , wherein the decrease in RCA is between 1% and 80%.

3 . A recombinant maize plant comprising a construct, wherein the construct comprises a polynucleotide encoding a sequence complementary to all of SEQ ID NO: 1 operably linked to a heterologous promoter, wherein expression of the sequence complementary to SEQ ID NO:1 in the recombinant maize plant decreases a function of a protein encoded by SEQ ID NO: 1 in the recombinant maize plant, wherein the recombinant maize plant exhibits decreased root cortical aerenchyma (RCA) as compared to a maize plant lacking the construct.

4 . The recombinant maize plant of claim 3 , wherein the decrease in RCA is between 1% and 80%.

Assignments (5)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 2, 2026
From: BENNETT, MALCOLM JOHN
To: THE UNIVERSITY OF NOTTINGHAM
Reel/Frame 073655/0866 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 29, 2026
From: LYNCH, JONATHAN; SAENGWILAI, PATOMPONG
To: THE PENN STATE RESEARCH FOUNDATION
Reel/Frame 073623/0744 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 11, 2025
From: LYNCH, JONATHAN P.
To: THE PENN STATE RESEARCH FOUNDATION
Reel/Frame 072854/0507 →
CONFIRMATORY LICENSE Recorded Sep 25, 2019
From: UNIVERSITY OF WISCONSIN, MADISON
To: NATIONAL SCIENCE FOUNDATION
Reel/Frame 050493/0743 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 4, 2019
From: KAEPPLER, SHAWN; JOHNSON, JAMES
To: WISCONSIN ALUMNI RESEARCH FOUNDATION
Reel/Frame 050257/0680 →
Continuity (3)
Continuation 15704502 · Sep 14, 2017
Provisional Application 62395434 · Sep 16, 2016
Related Publication 20190380295A1 · Dec 19, 2019
References Cited (36)
US 4683195A · Mullis et al. · 1987 [cited by applicant]
US 8030473B2 · Carrington et al. · 2011 [cited by applicant]
US 10995342B2 · Bent et al. · 2021 [cited by applicant]
US 11136591B2 · Ranjan et al. · 2021 [cited by applicant]
Maize Genetics and Genomics Database, UniformMu Transposon Resource, Mar. 2011 (Year: 2011). [cited by examiner]
Yibrah et al. Hereditas 118:273-2890 (Year: 1993). [cited by examiner]
Thomas et al. The Plant Journal 25(4):417-425 (Year: 2001). [cited by examiner]
Bates, D., Maechler, M., Bolker, B., and Walker, S. Fitting linear mixed-effects models using Ime4. Journal of Statistical Software. 67(1):1-48 (2014). [cited by applicant]
Burton, A. L., Williams, M., Lynch, J. P. & Brown, K. M. RootScan: Software for high-throughput analysis of root anatomical traits. Plant Soil 357, 189-203 (2012). [cited by applicant]
Burton, A. L. et al. QTL mapping and phenotypic variation of root anatomical traits in maize ( [cited by applicant]
Chimungu, J. G., Brown, K. M. & Lynch, J. P. Reduced root cortical cell file No. improves drought tolerance in maize. Plant Physiol. 166, 1943-1955 (2014). [cited by applicant]
Chimungu, J. G., Brown, K. M. & Lynch, J. P. Large root cortical cell size improves drought tolerance in maize. Plant Physiol. 166, 2166-2178 (2014). [cited by applicant]
Chimungu, J. G. et al. Utility of root cortical aerenchyma under water limited conditions in tropical maize ( [cited by applicant]
Dellaporta, S. L., et al. Molecular Cloning of the Maize R-nj allele by transposon tagging with Ac, Chromosome structure and function, (1988) p. 263-282. [cited by applicant]
Drew, M. C., He, C. J. & Morgan, P. W. Programmed cell death and aerenchyma formation in roots. Trends Plant Sci. 5, 123-127 (2000). [cited by applicant]
Gao, X., Starmer, J. & Martin, E. R. A multiple testing correction method for genetic association studies using correlated single nucleotide polymorphisms. Geneic Epidemiol. 32, 361-369 (2008). [cited by applicant]
Grassini, P., Eskridge, K. M. & Cassman, K. G. Distinguishing between yield advances and yield plateaus in historical crop production trends. Nat. Commun. 4, 2918 (2013). [cited by applicant]
Gunawardena, A. H. L. A. N. et al. Characterisation of programmed cell death during aerenchyma formation induced by ethylene or hypoxia in roots of maize ( [cited by applicant]
Hansey, C. N., Johnson, J. M., Sekhon, R. S., Kaeppler, S. M. & Leon, N. De. Genetic diversity of a maize association population with restricted phenology. Crop Sci. 51, 704-715 (2011). [cited by applicant]
Hirsch, C. N. et al. Insights into the maize pan-genome and pan-transcriptome. Plant Cell 26, 121-135 (2014). [cited by applicant]
Lipka, A. E. et al. GAPIT: genome association and prediction integrated tool. Bioinformatics 28, 2397-2399 (2012). [cited by applicant]
Lynch, J. P. Roots of the second green revolution. Aust. J. Bot. 55, 493-512 (2007). [cited by applicant]
Lynch, J. P. Root phenes that reduce the metabolic costs of soil exploration: opportunities for 21st century agriculture. Plant. Cell Environ. 1775-1784 (2014). [cited by applicant]
Mano, Y. & Omori, F. Flooding tolerance in interspecific introgression lines containing chromosome segments from leosinte ( [cited by applicant]
Mano, Y. et al. QTL mapping of root aerenchyma formation in seedlings of a maize x rare teosinte ‘ [cited by applicant]
Niu, N. et al. EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development In rice. Nat. Commun. 4, 1445 (2013). [cited by applicant]
Postma, J. A. & Lynch, J. P. Root cortical aerenchyma enhances growth of [cited by applicant]
Rajhi, I. et al. Identification of genes expressed in maize root cortical cells during lysigenous aerenchyma formation using laser microdissection and microarray analyses. New Phytol. 190, 351-368 (2011). [cited by applicant]
Richards & Passioura. A breeding program to reduce the diameter of the major xylem vessel in the seminal roots of wheat and its effect on grain yield in rain-fed environments. Aust. J. Agric. Res. 40, 943-950 (1989). [cited by applicant]
Saengwilai, P., Nord, E., Chimungu, J., Brown, K. & Lynch, J. Root cortical aerenchyma enhances nitrogen acquisition from low nitrogen soils in maize ( [cited by applicant]
Sekhon, R. S. et al. Genome-wide atlas of transcription during maize development. Plant J. 66, 553-563 (2011). [cited by applicant]
Stelpflug, S. C. et al. An expanded maize gene expression atlas based on RNA-sequencing and its use to explore root development. Plant Genome (2015). doi:10.3835. [cited by applicant]
Subbaiah, C. C. & Sachs, M. M. Molecular and cellular adaptations of maize to flooding stress. Ann. Bot. 90, 119-127 (2003). [cited by applicant]
Sutton, M. A. et al. Too much of a good thing. Nature 472, 159-161 (2011). [cited by applicant]
Takahashi, H., Yamauchi, T., Rajhi, I., Nishizawa, N. K. & Nakazono, M. Transcript profiles in cortical cells of maize primary root during ethylene-induced lysigenous aerenchyma formation under aerobic conditions. Ann. … [cited by applicant]
Trachsel, S., Kaeppler, S. M., Brown, K. M. & Lynch, J. P. Shovelomics: high throughput phenotyping of maize ( [cited by applicant]