Patent
A sorghum seed comprising in its genome at least one polynucleotide encoding a polypeptide having an alanine to tyrosine substitution at position 93 of the sorghum AHAS protein large subunit. The plant has increased resistance to one or more herbicides, for example from the imidazolinone group, as compared to wild-type sorghum plants. The sorghum plant may comprise in its genome, one, two, three or more copies of a polynucleotide encoding a mutated large subunit of sorghum AHAS or a sorghum AHAS polypeptide of the invention. In this context, the sorghum plant may be tolerant to any herbicide capable of inhibiting AHAS enzyme activity. For example, the sorghum plant may be tolerant to herbicides of the imidazolinones type, such as imazethapyr, imazapir, and imazapic or to herbicides of the sulfonylurea group.
1. A method for identifying a sorghum herbicide-resistant plant, comprising:
a) providing a nucleic acid sample from a non-transgenic sorghum plant;
b) amplifying a region corresponding to the AHAS gene present in said nucleic acid sample from a sorghum plant;
c) identifying a herbicide-resistant sorghum plant based on the presence of one mutation in the amplified nucleic acid sample, where said mutation confers resistance to herbicides from the imidazolinone group, wherein said one mutation comprises an Ala93Thr substitution in an encoded polypeptide, wherein said polypeptide has acetohydroxyacid synthase activity, and wherein the nucleic acid sample comprises SEQ ID No. 1; and
d) applying one or more imidazolinone herbicides to the identified herbicide-resistant sorghum plant, wherein the sorghum plant has increased resistance to one or more imidazolinone herbicides as compared to wild-type sorghum plants.