Method of producing 2′-fucosyllactose using recombinant
View Patent ↗Provided is a method of producing 2′-fucosyllactose including culturing in a medium supplemented with lactose a recombinant Corynebacterium glutamicum transformed to express α-1,2-fucosyltransferase, transformed to express GDP-D-mannose-4,6-dehydratase, transformed to express GDP-L-fucose synthase, and transformed to express lactose permease, wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase.
1. A method of producing 2′-fucosyllactose comprising
culturing in a medium supplemented with lactose a recombinant Corynebacterium glutamicum transformed to express α-1,2-fucosyltransferase, transformed to express GDP-Dmannose-4,6-dehydratase, transformed to express GDP-L-fucose synthase, and transformed to express lactose permease, and
isolating 2′-fucosyllactose from the medium,
wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase.
2. The method according to claim 1 , wherein the medium further comprises glucose.
3. The method according to claim 2 , wherein the production of the fucosyllactose is carried out by fed-batch culture comprising further supplying glucose or lactose.
4. The method according to claim 1 , wherein the recombinant Corynebacterium glutamicum is transformed to overexpress phosphomannomutase, and transformed to overexpress GTP-mannose-1-phosphate guanylyltransferase.