Direct electron transfer-type oxidoreductase-modified molecular recognition element
A molecular recognition element comprising a target molecule-recognizing portion, and a direct electron transfer-type oxidoreductase linked to the target molecule-recognizing portion.
1. A molecular recognition element comprising
(i) a target molecule-recognizing portion, wherein the target molecule is an antigen and the target molecule-recognizing portion is an antibody against the antigen, and
(ii) a direct electron transfer-type oxidoreductase linked to the target molecule-recognizing portion, wherein the oxidoreductase is a glucose dehydrogenase comprising a cytochrome-containing subunit of SEQ ID NO: 4.
2. The molecular recognition element according to claim 1 , wherein the target molecule-recognizing portion and the direct electron transfer-type oxidoreductase are linked by a cross-linker.
3. The molecular recognition element according to claim 1 , wherein the molecular recognition element comprises a fusion protein of the target molecule-recognizing portion and the direct electron transfer-type oxidoreductase.
4. The molecular recognition element according to claim 1 , wherein the glucose dehydrogenase further comprises a catalytic subunit and a regulatory submit.
5. A sensor comprising an electrode, and the molecular recognition element according to claim 1 , immobilized on the electrode.
6. The sensor according to claim 5 , wherein the molecular recognition element is immobilized on the electrode by means of a monolayer forming molecule.
7. A method for measuring a target molecule comprising introducing a sample containing a target molecule into the sensor according to claim 5 ; and detecting a signal based on the target molecule.
8. A reagent for measuring a target molecule comprising the molecular recognition element according to claim 1 .