IP Library › Granted Patent US 11,358,999
Granted Patent B2
US 11,358,999 · App. 16/592,656 · Granted Jun 14, 2022

IL-12 heterodimeric Fc-fusion proteins

Inventors: Matthew Bernett (Monrovia, CA); John R. Desjarlais (Pasadena, CA); Rajat Varma (Hamden, CT); Ke Liu (Glendora, CA); Nargess Hassanzadeh-Kiabi (Pasadena, CA); Rumana Rashid (Temple City, CA)
Assignee: Xencor, Inc.
C07K14/5434C07K19/00A61K38/00A61K38/208C07K1/18C07K2319/30
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 11,358,999
App. No.
16/592,656
Granted
Jun 14, 2022
Kind
B2
Abstract

The present invention provides novel IL-12 Fc fusion proteins, methods of making and using the same. The IL-12. Fc fusion proteins are useful for treatment of cancer and can be used in combination with checkpoint blockade.

Claims (8)

1. A heterodimeric Fc fusion protein comprising:

a) a first fusion protein comprising a variant IL-12p40 subunit domain and a first Fc domain, wherein said IL-12p40 subunit domain is covalently attached to the N-terminus of said first Fc domain, wherein said variant IL-12p40 subunit domain comprises the amino acid sequence of SEQ ID NO:363, wherein said first Fc domain comprises the amino acid sequence of SEQ ID NO:42, wherein said IL-12p40 subunit domain is covalently attached to the N-terminus of said first Fc domain using a domain linker comprising the amino acid sequence of SEQ ID NO:107; and

b) a second fusion protein comprising an IL-12p35 subunit domain and a second Fc domain, wherein said IL-12p35 subunit domain is covalently attached to the N-terminus of said Fc domain, wherein said IL-12p35 subunit domain comprises the amino acid sequence of SEQ ID NO:2, wherein said second Fc domain comprises the amino acid sequence of SEQ ID NO:41, and wherein said IL-12p35 subunit domain is covalently attached to the N-terminus of said second Fc domain using a domain linker having the amino acid sequence of SEQ ID NO:10,

wherein said first and said second Fc domains comprise modifications promoting heterodimerization of said first and said second Fc domains.

2. A heterodimeric Fc fusion protein comprising:

a) a first fusion protein comprising a variant IL-12p40 subunit domain and a first Fc domain, wherein said IL-12p40 subunit domain is covalently attached to the N-terminus of said first Fc domain, wherein said first fusion protein comprises the amino acid sequence of SEQ ID NO:382 further comprising the amino acid substitutions M428L/N434S; and

b) a second fusion protein comprising an IL-12p35 subunit domain and a second Fc domain, wherein said IL-12p35 subunit domain is covalently attached to the N-terminus of said Fc domain, wherein said second fusion protein comprises the amino acid sequence of SEQ ID NO:404 further comprising the amino acid substitutions M428L/N434S,

wherein said first and said second Fc domains comprise modifications promoting heterodimerization of said first and said second Fc domains.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 23, 2021
From: BERNETT, MATTHEW J.; DESJARLAIS, JOHN R.; VARMA, RAJAT; LIU, KE; HASSANZADEH-KIABI, NARGESS; RASHID, RUMANA
To: XENCOR, INC.
Reel/Frame 056025/0624 →
Continuity (4)
Provisional Application 62740813 · Oct 3, 2018
Provisional Application 62810038 · Feb 25, 2019
Provisional Application 62848512 · May 15, 2019
Related Publication 20200216509A1 · Jul 9, 2020
Cited By (4)
US 12,234,270 US 12,280,120 US 12,522,639 US 12,662,520