Carrier for automated microbiology sample processing
Carriers are provided for microbiological laboratory use, as are methods for their use. The carriers may be used to transport patient samples between laboratory stations and can be loaded into automated AST systems. In an aspect, a method of performing AST may include loading a tube comprising a patient sample onto a carrier. An AST panel may be loaded onto the carrier. The carrier may be conveyed to an automated inoculation assembly. The patient sample may be inoculated from the tube into the AST panel. The AST panel may be loaded into an automated AST system.
1. A carrier for antimicrobial susceptibility testing (AST) panels, fluid troughs, and sample tubes comprising:
(a) one or more first stage spaces for an AST panel, wherein each first stage space comprises
(i) a stage space raised from a surface of the carrier having a stage perimeter smaller than an underside perimeter of each panel, and
(ii) at least one protrusion within said first stage space and extending normal to the stage space for engaging said panel;
(b) one or more second stage spaces for a fluid trough, wherein each second stage space comprises
(i) a stage space raised from a surface of the carrier having a stage perimeter smaller than an underside perimeter of each fluid trough, and
(ii) at least one protrusion within said first stage space and extending normal to the stage space for engaging said trough; and
(c) one or more receptacles configured to accept a sample tube, wherein said receptacles are located on said carrier or said carrier further comprises at least one fluid trough comprising one or more receptacles configured to accept a sample tube,
wherein said first stage spaces, said second stage spaces and said receptacles are arranged on said carrier so that a fluid handling device is moveable directly between a panel and a tube without the need to pass over any other panel.
2. The carrier of claim 1 , wherein said first stage spaces, said second stage spaces and said receptacles are arranged on said carrier such that
(a) the one or more second stage spaces are adjacent to and between at least two first stage spaces, and wherein the fluid trough comprises the one or more receptacles;
(b) the one or more second stage spaces are adjacent to and between at least two first stage spaces, and wherein the one or more receptacles are disposed along a perimeter of the carrier and the one or more receptacles are adjacent to the one or more second stage spaces;
(c) the one or more second stage spaces are adjacent to and between at least two first stage spaces, and wherein the one or more receptacles are disposed between one of the at least two first stage spaces and a third stage space; or
(d) the one or more first stage spaces are adjacent to and between two second stage spaces, and wherein one of the two second stage spaces is adjacent to the one or more receptacles.
3. A method of performing antimicrobial susceptibility testing (AST), comprising the steps of:
loading a tube comprising a patient sample onto a carrier of claim 1 ;
loading an AST panel onto the carrier;
conveying the carrier to an automatic inoculator;
inoculating the patient sample from the tube into the AST panel; and
loading the AST panel into an automated AST system.
4. The method of claim 3 , further comprising the step of reloading the AST panel onto the carrier from the automated AST system following completion of an automated AST process, and conveying the carrier to a second laboratory station.
5. The method of claim 3 , further comprising:
reading and comparing identifying information from the tube and from the AST panel; and
storing the identifying information in a central database system that associates sample orders, accession numbers, panels and carriers.
6. The method of claim 5 , further comprising pausing the method if the identifying information from the tube does not correspond with the information from the AST panel.
7. The method of claim 3 , further comprising the step of reading identifying information from the carrier and at least one of the tube and the AST panel, and associating the identifying information of the carrier with the identifying information of at least one of the tube and the AST panel.
8. The method of claim 7 , wherein the step of reading identifying information comprises scanning a barcode, reading an RFID tag, or reading an encoded magnetic pattern.
9. An antimicrobial susceptibility testing (AST) system comprising:
one or more containers each containing an array of sample wells; and
a carrier of claim 1 disposed under the one or more containers.
10. The AST system of claim 9 , further comprising additional stage protrusions, each stage protrusion corresponding with a corner of one of the containers.
11. The AST system of claim 9 , further comprising a second stage space adjacent the first stage space.
12. The AST system of claim 9 , further comprising a vessel disposed on the carrier adjacent the one or more containers.
13. The AST system of claim 12 , wherein the vessel further comprises a plurality of vessel wells, each vessel well located within the vessel such that each vessel well is adjacent to a corresponding container such that no other container is between the corresponding container and the vessel well.
14. The AST system of claim 12 , further comprising a plurality of receptacles, each receptacle configured to accept a tube and each receptacle located within the vessel such that each receptacle is adjacent to a corresponding container such that no other container is between the corresponding container and the receptacle.
15. The AST system of claim 14 , wherein each receptacle of the plurality of receptacles comprises a pin portion extending into the carrier configured to align the vessel with respect to the carrier.
16. The AST system of claim 12 , further comprising an alignment member extending normal to the carrier and into the vessel.
17. The AST system of claim 9 , further comprising one or more slots disposed along an underside of the carrier configured to receive one or more arms for positioning the carrier.
18. A method of performing antimicrobial susceptibility testing (AST), comprising the steps of:
loading a patient sample onto a carrier of claim 1 ;
loading an AST panel onto the carrier;
loading the AST panel into an automated AST system; and
transferring a portion of the patient sample to the AST panel.
19. The method of claim 18 , further comprising identifying that the patient sample corresponds with the AST panel.
20. The method of claim 19 , wherein identifying includes scanning a code associated with the patient sample.
21. The method of claim 18 , wherein the patient sample is selected from the group consisting of a positive blood culture and an isolate.
22. The method of claim 18 , further comprising diluting the patient sample by supplying a reagent.