Diagnosis and treatment of viral diseases
Provided are methods of diagnosing a viral disease such as idiopathic pulmonary fibrosis, Castleman's disease, a lymphoma, a thymoma or a sarcoma in a patient by identifying one or more virus-specific elements such as a nucleic acid or a viral protein or a patient antibody to a virus-specific element, as well as to kits for diagnosing the viral disease in a patient. Further provided are methods of monitoring disease progression and/or the efficacy of therapy by measuring the levels of a virus-specific element in a sample from a patient, and methods of identifying therapeutic agents that show efficacy in reducing levels of virus-specific agents in vitro. Still further provided are methods of treating idiopathic pulmonary fibrosis, a lymphoproliferative disease and cancer, as well as to methods of preventing viral infection, including Herpesvirus saimiri infection.
1. A composition comprising a viral nucleic acid target sequence hybridized to
a non-radioactively labeled nucleic acid comprising one or more nucleic acid sequences complementary to Herpesvirus saimiri , wherein the non-radioactive label comprises a fluorescent label, a chemiluminescent label, a hapten label, a chromogenic label, or an energy transfer pair element, wherein the non-radioactively labeled nucleic acid is completely complementary to a sequence of at least 20 contiguous nucleotides within ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13 or SEQ ID NO: 14
and wherein the composition is present in a human cell in an isolated lung tissue specimen.
2. The composition of claim 1 , wherein the viral nucleic acid target sequence has the nucleotide sequence of Herpesvirus saimiri.
3. The composition of claim 1 , wherein the non-radioactively labeled nucleic acid is labeled with biotin.
4. The composition of claim 1 , wherein the non-radioactively labeled nucleic acid comprises at least one locked nucleic acid (LNA) oligonucleotide.
5. The composition of claim 1 , wherein the non-radioactively labeled nucleic acid comprises a nucleotide sequence of SEQ ID:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8.