Method of preparing a composition comprising ferulic acid
The present invention relates to a method of preparing a composition comprising ferulic acid. An aspect of the invention is a composition comprising hydrolysed wheat bran for use in the treatment or prevention of metabolic disease.
1. A method of preparing a composition comprising ferulic acid, the method comprising:
forming a bran mixture comprising bran and water, the bran mixture having a water content of at least 50 wt. % and comprising from 1 to 50% by weight of bran;
heating the bran mixture to a temperature of between 75 and 150° C.;
cooling the bran mixture to below 50° C.; and
subjecting the bran mixture to an enzymatic treatment comprising contacting the bran mixture with a carbohydrase and a feruloyl esterase.
2. The method according to claim 1 , wherein the bran mixture is heated by direct steam injection.
3. The method according to claim 2 , wherein the bran mixture is formed by enzymatic hydrolysis of a whole grain cereal with an alpha-amylase, the alpha-amylase showing no hydrolytic activity towards dietary fibers when in an active state.
4. The method according to claim 1 , wherein the bran mixture is heated to a temperature of between 100 and 150° C. after being subjected to the enzymatic treatment.
5. The method according to claim 1 , wherein the bran mixture is dried after being subjected to the enzymatic treatment.
6. The method according to claim 1 , wherein the bran has a particle size distribution D90 of less than 500 μm.
7. The method according to claim 1 , wherein the enzymatic treatment is performed at a temperature between 30 and 50° C.
8. The method according to claim 1 , wherein the bran is wheat bran.
9. The method according to claim 1 , wherein the enzymatic treatment is performed by micro-organisms.
10. The method according to claim 1 , wherein the bran mixture is mixed or assembled with a food ingredient after being subjected to the enzymatic treatment.
11. A method for treating metabolic disease or obesity, the method comprising administering a composition comprising hydrolysed wheat bran to an individual in need thereof, the composition comprising between 0.001 and 0.25 wt. % ferulic acid.
12. The method according to claim 11 , wherein the metabolic disease or obesity is selected from the group consisting of type II diabetes, and cardiovascular disease.
13. The method in accordance with claim 11 , wherein the composition is to be administered orally.
14. The method in accordance with claim 11 , wherein the composition is to be administered in a daily dose of between 0.30 and 3.0 mg/kg body weight per day ferulic acid.