Target substance detection chip, target substance detection device, and target substance detection method
A target substance detection device detecting a target substance using magnetic particles includes a detection chip, a light irradiation unit, and a magnetic field application unit, wherein the detection chip includes a light transmissive member having a sensing surface arranged on a surface constituting a top surface relative to a bottom surface, an inclined surface, and a main body portion capable of receiving light and guiding the light through the interior, the light transmissive member having a light directing structure in which the light applied from the top surface side and passed through the inclined surface is directed via the main body portion to the sensing surface under the condition of total reflection, the light irradiation unit is operable to irradiate the sensing surface with light under the condition of total reflection via the light directing structure, and the magnetic field application unit.
1. A target substance detection device comprising:
a detection chip including an entirely approximately plate-shaped light transmissive member, the light transmissive member comprising:
a sensing surface arranged on a surface constituting a top surface relative to a bottom surface,
an inclined surface which is one of
an upwardly inclined surface inclined away from the sensing surface from the top surface toward the bottom surface side with respect to a thickness direction and
a downwardly inclined surface inclined away from the sensing surface from the bottom surface toward the top surface side with respect to the thickness direction, and
a main body portion capable of receiving light and guiding the light through an interior thereof,
the light transmissive member having a light directing structure which is one of
a first light directing structure in which the light applied from the top surface side and passed through the upwardly inclined surface is directed via the main body portion to the sensing surface under a condition of total reflection and
a second light directing structure in which the light applied from the top surface side and reflected at the downwardly inclined surface is directed via the main body portion to the sensing surface under the condition of total reflection;
a light irradiation unit disposed on the side of the top surface of the light transmissive member and operable to irradiate the sensing surface with the light under the condition of total reflection via the light directing structure; and
a magnetic field application unit disposed on the side of the bottom surface of the light transmissive member;
wherein the light transmissive member comprises at least one notch portion out of a top surface side notch portion formed in the top surface and having the upwardly inclined surface and a bottom surface side notch portion formed in the bottom surface and having the downwardly inclined surface.
2. The target substance detection device according to claim 1 , wherein the notch portion is filled with a low refractive material that is lower in refractive index than the main body portion.
3. The target substance detection device according to claim 1 , wherein the light directing structure is operable to cause at least one of the light passed through the upwardly inclined surface in the first light directing structure and the light reflected at the downwardly inclined surface in the second light directing structure to be reflected at the bottom surface before being directed to the sensing surface under the condition of total reflection.
4. The target substance detection device according to claim 1 , wherein a shortest distance between a light incident position on the inclined surface and an irradiated position on the sensing surface is 1.0 mm to 50.0 mm.
5. The target substance detection device according to claim 1 , wherein the light transmissive member has a thickness of 0.1 mm to 10.0 mm.
6. The target substance detection device according to claim 1 , wherein the light transmissive member comprises a sample liquid storage groove formed in the top surface, the groove having at least a portion constituting the sensing surface.
7. The target substance detection device according to claim 6 , wherein the sample liquid storage groove comprises, as the sensing surface, an inclined sensing surface inclined away from the inclined surface from the top surface toward the bottom surface side with respect to the thickness direction of the light transmissive member.
8. The target substance detection device according to claim 1 , wherein the light transmissive member comprises a portion of the top surface constituting the sensing surface, and comprises a sidewall portion erected around the sensing surface to form a box-shaped body with the sensing surface as a bottom thereof.
9. A target substance detection method using the target substance detection device according to claim 1 , comprising:
a light irradiation step of irradiating the sensing surface with light under a condition of total reflection from the side of the top surface of the light transmissive member via the light directing structure; and
a magnetic field application step of applying a magnetic field from the side of the bottom surface of the light transmissive member.