Vitrification device and method for preparing sample
Provided herein are devices and related methods for rapidly freezing a sample using, for example, liquid nitrogen. The device includes an input portion with an input port, a sample chamber, a waste reservoir in fluid communication with the sample chamber, and a filtering mechanism that selectively allows a fluid introduced through the input port to pass through the sample chamber and into the waste reservoir, while retaining a sample within the sample chamber. The sample chamber, waste reservoir, and filtering mechanism are configured to draw fluid from the sample chamber through the filtering mechanism and into the waste reservoir via capillary action.
1 . A vitrification device comprising:
an input portion with an input port;
a sample chamber coupled to the input port, wherein the sample chamber comprises a magnet configured to engage a magnet of an additional vitrification device;
a waste reservoir coupled to the sample chamber and in fluid communication with the sample chamber such that the sample chamber is between the input port and the waste reservoir; and
a filtering mechanism positioned between the sample chamber and the waste reservoir such that the filtering mechanism is arranged to retain a sample within the sample chamber while allowing a fluid introduced through the input port to pass through the sample chamber, across the filtering mechanism, and into the waste reservoir;
wherein the sample chamber, waste reservoir, and filtering mechanism are configured to draw fluid from the sample chamber through the filtering mechanism and into the waste reservoir via capillary action.
2 . The vitrification device of claim 1 , further comprising at least one viewing window incorporated as a part of the sample chamber, where the at least one viewing window is constructed of different material than a remainder of the sample chamber, wherein the at least one viewing window is configured such that the sample within the sample chamber is viewable through the at least one viewing window.
3 . The vitrification device of claim 1 , further comprising a cap coupled to the input portion and rotatable around an axis of the input portion between a closed configuration in which the cap seals the input port so as to inhibit flow of fluid within the vitrification device and an open configuration in which fluid can be provided to the sample chamber through the input port.
4 . The vitrification device of claim 1 , wherein the filtering mechanism comprises a filtration grate comprising a plurality of alternating lower and taller segments thereby forming, at the lower segments, a plurality of microfluidic channels for promoting capillary action.
5 . The vitrification device of claim 1 , wherein the sample chamber is formed of at least one of: an acrylic-based material, a polypropylene-based material, a polycarbonate-based material and a copolyester-based material.
6 . The vitrification device of claim 1 , wherein the sample chamber has a wall with a thickness less than or equal to 0.002 inches.
7 . The vitrification device of claim 1 , further comprising a handle coupled to the input portion, wherein the waste reservoir is positioned in the handle.
8 . The vitrification device of claim 1 , wherein a fluid capacity of the waste reservoir is sized to hold all waste fluids resulting from use of the vitrification device for treating and preparing the sample for vitrification without emptying the waste reservoir.
9 . The vitrification device of claim 1 , further comprising a filter coupled to the waste reservoir and configured to allow air to vent from the waste reservoir such that the fluid can flow into the waste reservoir concurrently with retention of the fluid in the waste reservoir.
10 . A vitrification device comprising:
an input portion with an input port;
a sample chamber coupled to the input port:
a waste reservoir coupled to the sample chamber and in fluid communication with the sample chamber such that the sample chamber is between the input port and the waste reservoir; and
a filtering mechanism positioned between the sample chamber and the waste reservoir such that the filtering mechanism is arranged to retain a sample within the sample chamber while allowing a fluid introduced through the input port to pass through the sample chamber, across the filtering mechanism and into the waste reservoir;
wherein the sample chamber, waste reservoir, and filtering mechanism are configured to draw fluid from the sample chamber through the filtering mechanism and into the waste reservoir via capillary action;
wherein the filtering mechanism comprises a filtration grate positioned on an island between the sample chamber and an input portion of the waste reservoir, the island comprising a U-shaped capture pocket configured to capture and retain the sample, the vitrification device further comprising a bypass channel on a side of the island and extending between the sample chamber and the input portion of the waste reservoir.
11 . A method for preparing a sample, comprising:
delivering a sample into a sample chamber of a vitrification device and adjacent a filtering mechanism separating the sample chamber from a waste reservoir of the vitrification device, wherein the filtering mechanism is coupled to the sample chamber; and
treating the sample with a first fluid by receiving the first fluid via an input port, pushing the first fluid from the input port, through the sample chamber, across the filtering mechanism, and into the waste reservoir with a fluidic force, while the filtering mechanism retains the sample within the sample chamber;
wherein pushing the first fluid into the waste reservoir initiates capillary action in the filtering mechanism resulting in the capillary action drawing subsequent fluids through the sample chamber and into the waste reservoir;
wherein the filtering mechanism comprises a filtration grate positioned on an island between the sample chamber and an input portion of the waste reservoir, the island comprising a U-shaped capture pocket configured to capture and retain the sample, the vitrification device further comprising a bypass channel on a side of the island and extending between the sample chamber and the input portion of the waste reservoir.
12 . The method of claim 11 , further comprising treating the sample with a second fluid by drawing the second fluid through the sample chamber and into the waste reservoir via capillary action, while the filtering mechanism retains the sample within the sample chamber.
13 . The method of claim 12 , comprising holding, simultaneously by the waste reservoir, the first fluid and the second fluid within a handle of the vitrification device subsequent to the treating the sample with the first fluid and the treating the sample with the second fluid.
14 . The method of claim 11 , further comprising viewing the sample in the sample chamber through a viewing window in the sample chamber, the viewing window made of a different material than a remainder of the vitrification device.
15 . The method of claim 11 , further comprising vitrifying the sample in the sample chamber.
16 . The method of claim 15 , wherein the sample is vitrified by contacting the sample chamber with liquid nitrogen.
17 . The method of claim 15 , further comprising thawing the sample by contacting the sample chamber with a warm solution.
18 . The method of claim 11 , comprising holding, by the waste reservoir, the first fluid within a handle of the vitrification device subsequent to the treating the sample with the first fluid.