Anti-CRISPR genes and proteins and methods of use
The invention relates to anti-CRISPR genes and anti-CRISPR proteins, and their uses in various biotechnology applications.
1. A method to favor the screening of a bacterial mechanism providing resistance against a virulent phage other than a given class 2 type II CRISPR-Cas mediated resistance, comprising:
1) providing a bacterial strain, the genome of which contains a given class 2 type II CRISPR-Cas system, which is known to be active against target nucleic acids;
2) expressing in said bacterial strain a gene encoding a protein which interferes with a function of said given class 2 type II CRISPR-Cas system;
3) exposing said bacterial strain of step 2) to a virulent phage; and
4) selecting bacteriophage-insensitive mutants (BIMs),
wherein the resistance in said selected BIMs is provided by a bacterial mechanism other than the given class 2 type II CRISPR-Cas-mediated resistance.
2. The method of claim 1 , wherein said bacterial mechanism providing phage resistance to said BIMs selected in step 4) is a mechanism mediated by another CRISPR-Cas system.
3. A method to enrich a bacterial population in bacteriophage-insensitive mutants (BIMs) other than BIMs due to a given CRISPR-Cas system, comprising:
1) providing a bacterial population comprising a bacterial strain, the genome of which contains a given class 2 type II CRISPR-Cas system, which is known to be active against target nucleic acids;
2) expressing in said bacterial strain a gene encoding a protein which interferes with a function of said given class 2 type II CRISPR-Cas system;
3) exposing said bacterial strain of step 2) to a virulent phage; and
4) selecting BIMs,
wherein the selected BIMs have acquired resistance to said phage by a mechanism other than the given class 2 type II CRISPR-Cas system.
4. The method according to claim 1 , wherein said protein which interferes with a function of a given class 2 type II CRISPR-Cas system is a protein which interferes with the interference function of a class 2 type II CRISPR-Cas system and downmodulates the activity of a Cas9 protein component of said class 2 type II CRISPR-Cas system.
5. The method according to claim 4 , wherein said protein downmodulates the activity of a Cas9 protein of a Gram-positive bacterium.
6. The method according to claim 5 , wherein said protein downmodulates the activity of a Cas9 protein of a Streptococcus genus strain or a Streptococcus pyogenes strain.
7. The method according to claim 1 , wherein said protein which interferes with a function of said given class 2 type II CRISPR-Cas system is a protein, which has the sequence as defined in SEQ ID NO: 10 or has a sequence having at least 70% similarity with SEQ ID NO: 10 or has a sequence having at least 90% identity with SEQ ID NO: 10.
8. The method according to claim 1 , wherein said bacterial strain is a Gram-positive bacterial strain.
9. A method to downmodulate, in a bacterial strain, the activity of a class 2 type II CRISPR-Cas system against target nucleic acids, comprising expressing in said bacterial strain a gene encoding a protein which interferes with a function of said class 2 type II CRISPR-Cas system, wherein said gene encodes a protein having at least 70% similarity or at least 90% identity to the sequence defined in SEQ ID NO: 10.
10. The method according to claim 3 , wherein said protein which interferes with a function of a given class 2 type II CRISPR-Cas system is a protein which interferes with the interference function of a class 2 type II CRISPR-Cas system and downmodulates the activity of a Cas9 protein component of said class 2 type II CRISPR-Cas system.
11. The method according to claim 10 , wherein said protein downmodulates the activity of a Cas9 protein of a Gram-positive bacterium.
12. The method according to claim 11 , wherein said protein downmodulates the activity of a Cas9 protein of a Streptococcus genus strain or a Streptococcus pyogenes strain.
13. The method according to claim 3 , wherein said protein which interferes with a function of said given class 2 type II CRISPR-Cas system is a protein, which has the sequence as defined in SEQ ID NO: 10 or has a sequence having at least 70% similarity with SEQ ID NO: 10 or has a sequence having at least 90% identity with SEQ ID NO: 10.
14. The method according to claim 3 , wherein said bacterial strain is a Gram-positive bacterial strain.