Polypeptide solution, production method for polypeptide fiber, and artificial polypeptide
The present invention relates to a polypeptide solution in which a polypeptide including an amino acid sequence derived from type I keratin that belongs to Cluster Ia is dissolved in a solvent including a formic acid or a solvent including an aprotic polar agent having a dipole moment of 3.0 D or more and an inorganic salt.
1. A polypeptide solution in which an artificial polypeptide including the amino acid sequence selected from the group consisting of the amino acid sequences having 85% or more sequence identity with the amino acid sequences represented by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5 is dissolved in a solvent including a formic acid or a solvent including an aprotic polar agent having a dipole moment of 3.0 D or more and an inorganic salt, wherein the artificial polypeptide is expressed by a host transformed with an expression vector, and wherein the host is prokaryotic organism.
2. The polypeptide solution according to claim 1 ,
wherein the solvent including an aprotic polar agent having a dipole moment of 3.0 D or more and an inorganic salt further includes a reducing agent.
3. The polypeptide solution according to claim 2 ,
wherein the reducing agent is at least one selected from the group consisting of thiols, tris(2-carboxyethyl)phosphine hydrochloride, tris(hydroxypropyl)phosphine, and sodium pyrosulfite.
4. The polypeptide solution according to claim 3 ,
wherein the thiols are at least one selected from the group consisting of dithiothreitol, β-mercaptoethanol, 3-mercapto-1,2-propandiol, 1,2-ethanthiol, thioglycolic acid, and ammonium thioglycolate (ATG).
5. The polypeptide solution according to claim 1 ,
wherein the aprotic polar agent having a dipole moment of 3.0 D or more is at least one selected from the group consisting of dimethyl sulfoxide, N,N-dimethylformamide, N,N-dimethylacetamide, 1,3-dimethyl-2-imidazolidone, N-methyl-2-pyrrolidone, and acetonitrile.
6. The polypeptide solution according to claim 1 ,
wherein the inorganic salt is at least one selected from the group consisting of alkaline metal halide, alkaline earth metal halide, alkaline earth metal nitrate, sodium thiocyanate, and ammonium thiocyanate (guanidium thiocyanate).
7. A production method for polypeptide fiber using the polypeptide solution according to claim 1 , the method comprising:
a step of using the polypeptide solution as a dope solution, extruding the dope solution from an orifice to a coagulation solution, and obtaining an undrawn yarn.
8. The production method for polypeptide fiber according to claim 7 , further comprising:
a step of drawing the undrawn yarn.
9. The production method for polypeptide fiber according to claim 7 ,
wherein the coagulation solution is at least one selected from the group consisting of methanol, ethanol, and 2-propanol.
10. The polypeptide solution according to claim 1 , wherein the sequence identity is 90% or more.
11. The polypeptide solution according to claim 1 , wherein the sequence identity is 95% or more.
12. The polypeptide solution according to claim 1 , wherein the sequence identity is 98% or more.
13. The polypeptide solution according to claim 1 , wherein the sequence identity is 99% or more.
14. The polypeptide solution according to claim 1 , wherein the artificial polypeptide includes the amino acid sequence selected from the group consisting of the amino acid sequences represented by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5.