Optimizing method of suction condition of microparticle and microparticle fractionating device
To provide a technology of optimizing a suction condition of a microparticle. The present technology provides an optimizing method of a suction condition of a microparticle including: a particle number counting step of detecting a time point when a microparticle passes through a predetermined position on a main flow path through which liquid containing the microparticle flows, sucking the microparticle from the main flow path to a microparticle suction flow path by the microparticle suction flow path with a predetermined suction force, and counting the number of microparticles sucked into the microparticle suction flow path; and a step of determining an elapsed time from passage through the predetermined position with which the suction by the microparticle suction flow path should be performed on the basis of a time from the time point when the microparticle passes through the predetermined position on the main flow path until the suction is performed and the number of counted microparticles.
1. An optimizing method of a suction condition of a microparticle comprising:
a particle number counting step of
detecting a time point when a microparticle passes through a predetermined position on a main flow path through which liquid containing the microparticle flows,
sucking the microparticle from the main flow path to a microparticle suction flow path by providing the microparticle suction flow path with a predetermined suction force, and
counting the number of microparticles sucked into the microparticle suction flow path; and
a step of determining an elapsed time from passage through the predetermined position with which the suction by the microparticle suction flow path should be performed on a basis of a time from the time point when the microparticle passes through the predetermined position on the main flow path until the suction is performed and the number of counted microparticles.
2. The method according to claim 1 , further comprising:
a repeating step of repeating the particle number counting step while changing the time from the time point when the microparticle passes through the predetermined position on the main flow path until the suction is performed.
3. The method according to claim 2 , further comprising:
a second repeating step of repeating the particle number counting step and/or the repeating step while changing the suction force.
4. The method according to claim 3 ,
wherein, at the second repeating step, the suction force is decreased stepwise at a predetermined rate, and the second repeating step is performed until a result in which the number of microparticles sucked into the microparticle suction flow path is 0 in every case of elapsed time is obtained.
5. The method according to claim 4 ,
wherein, at the determining step, the suction force obtained by increasing at a predetermined rate from the suction force until a desired number of particles has been sucked into the microparticle suction flow path is determined as the suction force which should be applied to the suction of the microparticle.
6. The method according to claim 2 ,
wherein, at the determining step, a rate of the suction to the microparticle suction flow path of the microparticles is calculated on a basis of the number of microparticles counted at the particle number counting step and the repeating step, and an elapsed time from the passage through the predetermined position with which the suction by the microparticle suction flow path should be performed is determined on a basis of the rate.
7. The method according to claim 1 ,
wherein, at the particle number counting step, the number of microparticles is counted in a predetermined position in the microparticle suction flow path.
8. The method according to claim 1 ,
wherein, at the particle number counting step, the number of microparticles is counted by detecting passage through a predetermined position in the microparticle suction flow path.
9. The method according to claim 1 performed for optimizing the suction condition of the microparticle in a microchip including the main flow path through which the liquid containing the microparticle flows, the microparticle suction flow path coaxial with the main flow path, and a branch flow path branching from the main flow path.
10. A microparticle fractionating device comprising:
a control unit which executes a particle number counting step of
detecting a time point when a microparticle passes through a predetermined position on a main flow path through which liquid containing the microparticle flows,
sucking the microparticle from the main flow path to a microparticle suction flow path by providing the microparticle suction flow path with a predetermined suction force, and
counting the number of microparticles sucked into the microparticle suction flow path; and
a determining unit which determines an elapsed time from passage through the predetermined position with which the suction by the microparticle suction flow path should be performed on a basis of a time from the time point when the microparticle passes through the predetermined position on the main flow path until the suction is performed and the number of counted microparticles.