Cell culturing method and device
A cell culturing method using a cell culturing bag ( 1 ) in which a plurality of recess portions are formed, including: a bag body ( 2 ) configured from an upper film ( 21 ) and a lower film ( 22 ) which seal a peripheral portion; and a port ( 3 ) mounted on the bag body ( 2 ), wherein the cell culturing bag ( 1 ) is placed on a placement surface ( 5 a ) of a stand ( 5 ), and while a culture medium (S) is being injected or discharged from the port ( 3 ), a pressing member ( 6 ) having a bottom surface ( 6 a ) substantially parallel to the placement surface ( 5 a ) is used to press the upper film ( 21 ) from above.
1. A method for culturing cells, comprising:
placing a cell culturing bag on a placement surface of a stand, wherein the cell culturing bag comprises a bag body configured from an upper film and a lower film which are sealed at a peripheral portion, and a port mounted on the bag body, wherein a plurality of recess portions each serving as a cell culture portion are formed in the lower film, wherein the upper film has a swelling shape which is swollen into a plateau shape configured from a flat top surface part; and rising parts standing from the lower film with predetermined height formed on a circumference of the top surface part, and wherein a plurality of openings or recess portions are formed on the placement surface of the stand in positions corresponding to the plurality of recess portions of the lower film as a shape for receiving each of the plurality of recess portions of the lower film;
inseminating the cells into the bag body to provide a number of the cells in each recess portion so that a spheroid having an appropriate size is formed from the cells provided in each recess portion;
discharging a culture medium from the port while simultaneously confining the cells or the spheroid in the plurality of recess portions; and
the confining occurring by descending the pressing member to press the top surface part of the upper film from above, the pressing member having a bottom surface parallel to the placement surface, wherein the bottom surface of the pressing member is brought into contact with the top surface part of the upper film, and the pressing member descending until the upper film closes the plurality of recess portions.
2. The method for culturing cells according to claim 1 , wherein the bottom surface of the pressing member has: plane portions in positions presenting over peripheral edges of the plurality of recess portions; and projection portions projected from the plane portions in positions presenting over the plurality of recess portions.
3. The method for culturing cells according to claim 1 , wherein the culture medium is injected into the port and/or discharged from the port by an injection and discharge means connected to the port while the pressing member is being used to press the upper film.
4. The method for culturing cells according to claim 1 , wherein, upon injecting or discharging the culture medium from the port, a flow rate is controlled in such a manner that, as a height of the upper film or the pressing member is lowered, a flow rate is adjusted to be slower.