Glucose sensitive compositions for drug delivery

1. A glucose sensitive drug delivery system comprising a core comprising an active agent and a polymeric shell encapsulating the core, wherein the core is attached to the polymeric shell via a plurality of boronate esters having the structure:

wherein R 1 , R 2 , R 3 , and R 4 are independently selected from hydrogen, C 1-8 alkyl, C 2-8 alkenyl, C 2-8 alkynyl, aryl, heteroaryl, C 3-8 cycloalkyl, and C 1-8 heteroaryl, which may be substituted or unsubstituted, and wherein any two or more of R 1 , R 2 , R 3 , and R 4 may together form a ring.

2. The drug delivery system of claim 1 , wherein R 1 and R 3 are hydrogen and R 2 and R 4 together form a phenyl ring.

3. The drug delivery system of claim 1 , wherein the polymeric shell comprises a carboxylate-bearing polymer selected from the group consisting of hyaluronic acid, poly(α-glutamic acid), poly(α-glutamic acid), poly(aspartic acid), chondroitin sulfate, carboxymethylcellulose, and combinations thereof, wherein at least a portion of the carboxylate groups are modified to contain the plurality of boronate esters.

4. The drug delivery system of claim 3 , wherein the polymeric shell comprises hyaluronic acid.

5. The drug delivery system of claim 3 , wherein the core comprises a liposome loaded with a small molecule drug or therapeutic protein.

6. The drug delivery system of claim 1 , wherein the active agent comprises as regular insulin, insulin degludec, insulin lispro, insulin aspart, insulin glulisine, insulin detemir, insulin glargine, NPH insulin, animal insulin, or a combination thereof.

7. The drug delivery system of claim 5 , wherein the liposome comprises a choline lipid component and an ethanolamine lipid component.

8. The drug delivery system of claim 5 , wherein the liposome comprises cholesterol, one or more cholesterol derivatives, or a combination thereof.

9. The drug delivery system of claim 5 , wherein the liposome comprises one or more primary lipids selected from phosphatidic acid, phosphatidylcholine, phosphatidylglycerol, phophatidylethanolamine phophatidylinositol, and phosphatidylserine, sphingomyelin, lecithin, lysolecithin, lysophosphatidylethanolamine, cerebrosides, diarachidoylphosphatidylcholine, didecanoyl-L-alpha-phosphatidylcholine, dielaidoylphosphatidylcholine, dilauroylphosphatidylcholine, dilinoleoylphosphatidylcholine, dimyristoylphosphatidylcholine dioleoylphosphatidylcholine, dipalmitoylphosphatidylcholine, distearoylphosphatidylcholine, 1-palmitoyl-2-oleoyl-phosphatidylcholine, diarachidoylphosphatidylglycerol, didecanoyl-L-alpha-phosphatidylglycerol, dielaidoylphosphatidylglycerol, dilauroylphosphatidylglycerol, dilinoleoylphosphatidylglycerol, dimyristoylphosphatidylglycerol, dioleoylphosphatidylglycerol, dipalmitoylphosphatidylglycerol, distearoylphosphatidylglycerol, 1-palmitoyl-2-oleoyl-phosphatidylglycerol, diarachidoylphosphatidylethanolamine, didecanoyl-L-alpha-phosphatidylethanolamine, dielaidoylphosphatidylethanolamine, dilauroylphosphatidylethanolamine, dilinoleoylphosphatidylethanolamine, dimyristoylphosphatidylethanolamine, dioleoylphosphatidylethanolamine, dipalmitoylphosphatidylethanolamine, distearoylphosphatidylethanolamine, 1-palmitoyl-2-oleoyl-phosphatidylethanolamine, diarachidoylphosphatidylinositol, didecanoyl-L-alpha-phosphatidylinositol, dielaidoylphosphatidylinositol, dilauroylphosphatidylinositol, dilinoleoylphosphatidylinositol, dimnyristoylphosphatidylinositol, dioleoylphosphatidylinositol, dipalmitoylphosphatidylinositol, distearoylphosphatidylinositol, 1-palmitoyl-2-olcoyl-phosphatidylinositol, diarachidoylphosphatidylserine, didecanoyl-L-alpha-phosphatidylserine, dielaidoylphosphatidylserine, dilauroylphosphatidylserine, dilinoleoylphosphatidylserine, dimyristoylphosphatidylserine, dioleoylphosphatidylserine, dipalmitoylphosphatidylserine, distearoylphosphatidylserine, 1-palmitoyl-2-olcoyl-phosphatidylserine, diarachidoyl sphingomyelin, didecanoyl sphingomyelin, dielaidoyl sphingomyelin, dilauroyl sphingomyelin, dilinoleoyl sphingomyelin, dimyristoyl sphingomyelin, sphingomyelin, dioleoyl sphingomyelin, dipalmitoyl sphingomyelin, distearoyl sphingomyelin, 1-palmitoyl-2-oleoyl-sphingomyelin, or a combination thereof.

10. The drug delivery system of claim 5 , wherein the liposome comprises a polymer anchor bonded to a boronate ester, having the formula:

wherein R a and R b are independently selected from C 6-32 alkyl-C(O)— or C 6-32 alkenyl-C(O)—, n is an integer from 5-1,000, Z and Z 1 are:

wherein R 1 , R 2 , R 3 , and R 4 are independently selected from hydrogen, C 1-8 alkyl, C 2-8 alkenyl, C 2-8 alkynyl, aryl, heteroaryl, C 3-8 cycloalkyl, and C 1-8 heteroaryl, which may be substituted or unsubstituted, and wherein any two or more of R 1 , R 2 , R 3 , and R 4 may together form a ring; n′ is an integer from 0-10, m is an integer from 0-3, and R c is independently selected from R c1 , OR c1 , N(R c1 ) 2 , SiR c1 3 , SR c1 , SO 2 R c1 , SO 2 N(R c1 ) 2 , C(O)R c1 ; C(O)OR c1 , OCOR c1 ; C(O)N(R c1 ) 2 , OC(O)N(R c1 ) 2 , N(R c1 )C(O)N(R c1 ) 2 , F, Cl, Br, I, cyano, and nitro, wherein R c1 is in each case independently selected from hydrogen, C 1-8 alkyl, C 2-8 alkenyl, C 2-8 alkynyl, aryl, C 1-8 heteroaryl, C 3-8 cycloalkyl, or C 1-8 heterocyclyl; and wherein any two or more R c groups may together form a ring.

11. The drug delivery system of claim 5 , wherein the liposome comprises a targeting factor.

12. The drug delivery system of claim 11 , wherein the targeting factor comprises human IgG Fc fragments, transferrin (Tf), or anti-intercellular adhesion molecule-1 (ICAM-1).

13. The drug delivery system of claim 1 , wherein the core is liberated from the polymeric shell in the presence of glucose.

14. A method of treating a hyperglycemic disorder, comprising administering to a patient in need thereof the drug delivery system of claim 6 .

15. A method of preparing a drug delivery system, comprising preparing a dry mixture of primary lipids and one or more anchor polymers; combining the dry mixture with an aqueous composition comprising an active agent to provide a liposome; and encapsulating the liposome with a polymer comprising a plurality of boronic esters.

16. The method of claim 15 , wherein the anchor polymer has the formula:

wherein R a and R b are independently selected from C 6-32 alkyl-C(O)—, or C 6-32 alkenyl-C(O)—, n is an integer from 5-1,000, and X and X 1 are diol bearing group.

17. The method of claim 16 , wherein X and X 1 are a moiety having the formula:

wherein n is an integer from 0-10, m is an integer from 0-3, and R c is independently selected from R c1 , OR c1 , N(R c1 ) 2 , SiR c1 3 , SR c1 , SO 2 R c1 , SO 2 N(R c1 ) 2 , C(O)R c1 ; C(O)OR c1 , OCOR c1 ; C(O)N(R c1 ) 2 , OC(O)N(R c1 ) 2 , N(R c1 )C(O)N(R c1 ) 2 , F, Cl, Br, I, cyano, and nitro, wherein R c1 is in each case independently selected from hydrogen, C 1-8 alkyl, C 2-8 alkenyl, C 2-8 alkynyl, aryl, C 1-8 heteroaryl, C 3-8 cycloalkyl, or C 1-8 heterocyclyl; and wherein any two or more R c groups may together form a ring.

18. The method of claim 15 , wherein the dry mixture further comprises one or more factor anchors, further comprising the step of conjugating a targeting factor to the factor anchor.

19. The method of claim 15 , wherein the polymer comprising a plurality of boronic esters is a hyaluronic acid polymer comprising a plurality of subunits having the structure:

wherein R 1 , R 2 , R 3 , and R 4 are independently selected from hydrogen, C 1-8 alkyl, C 2-8 alkenyl, C 2-8 alkynyl, aryl, heteroaryl, C 3-8 cycloalkyl, and C 1-8 heteroaryl, which may be substituted or unsubstituted, and wherein any two or more of R 1 , R 2 , R 3 , and R 4 may together form a ring, and unmodified subunits having the structure:

20. A drug delivery system, preparing by the process of claim 15 .