PDE5A destabilizing domains
Disclosed herein are systems, methods, and compositions for rapidly and reversibly destabilizing a target protein in vitro or in vivo, in the presence or absence of a cell-permeable, synthetic molecule or ligand.
1. A conditional protein stability system, comprising:
a nucleic acid sequence encoding a fusion protein comprising a protein of interest fused in-frame to a single-protein, ligand dependent destabilizing domain wherein the ligand dependent destabilizing domain comprises SEQ ID NO: 19 (encoded by SEQ ID NO: 36); and
a ligand, wherein the ligand is Sildenafil or Vardenafil,
wherein upon introduction of the nucleic acid sequence to a cell,
the fusion protein is expressed, and
wherein degradation of the protein of interest is decreased upon administration of the ligand.
2. The conditional protein stability system of claim 1 , wherein the ligand dependent destabilizing domain consists of SEQ ID NO: 19 (encoded by SEQ ID NO: 36).