Flow cells
An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.
1. A flow cell, comprising:
a substrate;
a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and
a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.
2. The flow cell as defined in claim 1 , wherein:
the first region includes a material having a first functional group; and
the second region includes a material having a second functional group that is different than the first functional group.
3. The flow cell as defined in claim 1 , further comprising a gap separating the first primer set from the second primer set.
4. The flow cell as defined in claim 1 , wherein:
the substrate includes depressions separated by interstitial regions; and
each of the depressions includes:
the first region located at a first portion; and
the second region located at a second portion.
5. The flow cell as defined in claim 4 , further comprising a gap separating the first region from the second region.
6. The flow cell as defined in claim 4 , wherein the first region and the second region partially overlap.
7. The flow cell as defined in claim 4 , wherein the first and second portions have different depths.
8. The flow cell as defined in claim 4 , wherein the first and second regions are different blocks of a block co-polymer.
9. The flow cell as defined in claim 1 , wherein:
the substrate includes depressions separated by interstitial regions;
each of the depressions includes the first region; and
the second region is located on at least some of the interstitial regions.
10. The flow cell as defined in claim 1 , wherein:
the first region includes a first polymer and the first primer set is grafted to the first polymer; and
the second region includes a second polymer and the second primer set is grafted to the second polymer.
11. The flow cell as defined in claim 10 , wherein the flow cell further comprises a protective coating on the first primer set and on the first polymer.
12. The flow cell as defined in claim 10 , wherein:
the first polymer is a first layer on the substrate;
the second polymer is a second layer on the first layer;
the flow cell further comprises:
a passivation resin on the second layer; and
features defined in the passivation resin, the second polymer and the first polymer; and
each of the first and second primer sets is exposed at each of the features.
13. The flow cell as defined in claim 1 , wherein:
the substrate includes depressions separated by interstitial regions;
each of the depressions includes:
a first portion where the first region is located; and
a second portion; and
the flow cell further comprises a bead located in the second portion, wherein the second region is at a surface of the bead.
14. The flow cell as defined in claim 1 , wherein the cleavable first primer includes a first cleavage site, the cleavable second primer includes a second cleavage site, and the first and second cleavage sites are of an identical type.
15. The flow cell as defined in claim 14 , wherein:
each of the un-cleavable first primer, the cleavable second primer, the cleavable first primer, and the cleavable second primer includes a respective linker;
the first cleavage site of the first cleavable primer is located along its linker; and
the second cleavage site of the second cleavable primer is located along its linker.
16. The flow cell as defined in claim 1 , wherein the cleavable first primer includes a first cleavage site, the cleavable second primer includes a second cleavage site, and the first and second cleavage sites are of a different type.
17. The flow cell as defined in claim 16 , wherein:
each of the un-cleavable first primer, the cleavable second primer, the cleavable first primer, and the cleavable second primer includes a respective linker;
the first cleavage site of the first cleavable primer is located along its linker; and
the second cleavage site of the second cleavable primer is located along its linker.
18. The flow cell as defined in claim 1 , wherein:
the first primer set is attached to a first support structure;
the first region is a first capture site that is attached to the first support structure;
the second primer set is attached to a second support structure that is different than the first support structure; and
the second region is a second capture site that is attached to the second support structure.
19. A flow cell, comprising:
a first substrate;
a first primer set attached to the first substrate, the first primer set including an un-cleavable first primer and a cleavable second primer;
a second substrate opposed to the first substrate; and
a second primer set attached to the second substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.
20. A kit, comprising:
a flow cell including:
a substrate including depressions separated by interstitial regions;
a first polymer layer in each of the depressions, wherein some functional groups of the first polymer layer are capped;
a first primer set attached to other functional groups of first polymer layer in each of the depressions; and
a second polymer layer on the interstitial regions; and
a priming fluid including:
a fluid carrier; and
a second primer set that is different from the first primer set;
wherein:
the first primer set includes an un-cleavable first primer and a cleavable second primer; and
the second primer set includes a cleavable first primer and an un-cleavable second primer.
21. A method, comprising:
introducing a template fluid to a flow cell including:
a substrate including depressions separated by interstitial regions;
a first polymer layer in each of the depressions, wherein exposed functional groups of the first polymer layer are capped;
a first primer set attached to the first polymer layer in each of the depressions, the first primer set including a cleavable first primer and an un-cleavable second primer;
and
a second polymer layer on the interstitial regions;
whereby a template from the template fluid is amplified to form a cluster in at least some of the depressions;
introducing a priming fluid, including an un-cleavable first primer and a cleavable second primer, to the flow cell, whereby the un-cleavable first primer and the cleavable second primer graft to the second polymer layer; and
initiating bridge amplification from the cluster to the un-cleavable first primer and a cleavable second primer, thereby forming a second cluster on at least some of the interstitial regions.
22. A method, comprising:
introducing a template fluid to a flow cell including:
a substrate including depressions separated by interstitial regions;
a first polymer layer in each of the depressions;
a first primer set attached to the first polymer layer, the first primer set including a cleavable first primer and an un-cleavable second primer;
an optional protective coating layer on the first polymer layer and on the first primer set;
a second polymer layer on the interstitial regions; and
a second primer set attached to the second polymer layer, the second primer set including an un-cleavable first primer and a cleavable second primer;
whereby a template from the template fluid is amplified to form a cluster in at least some of the depressions and on at least some of the interstitial regions; and
cleaving the cleavable first primer and the cleavable second primer.