Chimeric antibodies for treatment of amyloid deposition diseases
A chimeric mouse-human antibody for treatment of amyloid deposition diseases, pharmaceutical compositions comprising the antibody, methods and materials for producing the antibody, and methods for treating an amyloid deposition disease using the antibody and the pharmaceutical composition.
1. A chimeric mouse-human antibody for treating primary amyloidosis comprising a VK and VH region, wherein the VK region is SEQ ID NO: 47 and the VH region is SEQ ID NO: 48, wherein the chimeric antibody binds to amyloid fibrils with a higher affinity than its murine equivalent.
2. The chimeric mouse-human antibody of claim 1 which binds to an epitope expressed by the β-pleated sheet configuration of amyloid fibrils with higher affinity than the mouse antibody comprising the VK region of SEQ ID NO: 36 and the VH region of SEQ ID NO: 35.
3. A pharmaceutical composition comprising the antibody of claim 1 and a pharmaceutically acceptable carrier.
4. A method of treating an amyloid deposition disease in a human patient in need of such treatment which comprises administering to the patient the antibody of claim 1 together with a pharmaceutically acceptable carrier, in an amount effective to treat said amyloid deposition disease.
5. The method of claim 4 wherein the amyloid deposition disease is primary amyloidosis.
6. A method of detecting the presence of amyloid deposits in a patient suspected of having such deposits which comprises administering to the patient an amount of the antibody of claim 1 having a detectable label attached thereto, the amount of antibody administered being sufficient to allow detection of amyloid deposits if such are present.
7. The method of claim 6 wherein the detectable label is 1241.
8. A polypeptide selected from SEQ. ID NO: 47 and SEQ. ID NO: 48.