Uses of a saponin and method for its isolation
The present invention relates to the novel use of saponins having acetyl residues on one of their sugar residues. These saponins are able to enhance the transfection efficiency to a surprisingly much higher extent than already known saponins and even than Lipofectamin.
1. A method for an in vitro delivery of a nucleic acid, a lipid, a peptide and/or a protein to a cell, the method comprising the step of incubating the cell with the nucleic acid, the lipid, the peptide and/or the protein with a saponin according to formula (I):
wherein
R 1 is a xylose residue or an arabinose residue bonded with its C1 atom to the corresponding xylose residue of formula (I); and
R 2 is independent from other R 2 residues in the same molecule H or an acetyl residue, with the proviso that at least two acetyl residues are present in the saponin.
2. The method according to claim 1 , wherein the cell is a eukaryotic cell.
3. A transfection composition, comprising at least one transfection reagent chosen from the group consisting of liposomal-based transfection reagents and polymer-based transfection reagents as well as a saponin according to formula (I):
wherein
R 1 is a xylose residue or an arabinose residue bonded with its C1 atom to the corresponding xylose residue of formula (I); and
R 2 is independent from other R 2 residues in the same molecule H or an acetyl residue, with the proviso that at least two acetyl residues are present in the saponin.
4. The transfection composition according to claim 3 , wherein R 1 is a xylose residue and/or the saponin carries exactly two acetyl groups.
5. The transfection composition according to claim 4 , wherein the acetyl groups are bonded to the oxygen atoms in C3 and C4 position of the corresponding quinovose residue.
6. A method for an in vitro transfection, comprising the step of incubating a cell with a nucleic acid in the presence of a saponin according to formula (I):
wherein
R 1 is a xylose residue or an arabinose residue bonded with its C1 atom to the corresponding xylose residue of formula (I); and
R 2 is independent from other R 2 residues in the same molecule H or an acetyl residue, with the proviso that at least two acetyl residues are present in the saponin.
7. The method according to claim 6 , wherein the cell is a eukaryotic cell.
8. The method according to claim 6 , wherein the nucleic acid forms part of a nanoparticle.
9. The method according to claim 6 , wherein the saponin is used in a concentration lying in a range of 1 ng/mL to 25 ng/mL.
10. The method according to claim 6 , wherein the saponin is used in combination with at least one transfection reagent chosen from the group consisting of liposomal-based transfection reagents and polymer-based transfection reagents.
11. A method for isolating a saponin according to formula (I):
wherein
R 1 is a xylose residue or an arabinose residue bonded with its C1 atom to the corresponding xylose residue of formula (I); and
R 2 is independent from other R 2 residues in the same molecule H or an acetyl residue, with the proviso that at least two acetyl residues are present in the saponin
from Gypsophila elegans M. Bieb, comprising the following steps:
cutting roots of Gypsophila elegans M. Bieb,
freeze-drying and grinding the cut roots to obtain a root powder,
extracting the root powder with a high-concentrated organic solvent to obtain a root extract,
removing the high-concentrated organic solvent from the root extract to obtain a dry extract,
dissolving the dry extract in a low-concentrated organic solvent to obtain an extract solution,
subjecting the extract solution to at least one chromatographic separation step to obtain a purified saponin solution, and
removing any solvent from the purified saponin solution to obtain a purified saponin powder.