Bioconjugates with chemically modified backbones
Provided herein are synthetic bioconjugates comprising collagen-binding peptides covalently bound to chemically modified glycan backbones, compositions containing the same, and uses thereof.
1. A bioconjugate comprising at least one peptide(s), wherein the at least one peptide(s) comprises a collagen-binding unit covalently bonded to a chemically sulfated hyaluronic acid,
wherein the chemically sulfated hyaluronic acid has a molecular weight greater than about 150 kDa,
wherein the chemically sulfated hyaluronic acid comprises from 15 to 30 percent (%) functionalization with peptides,
wherein the chemically sulfated hyaluronic acid has been chemically sulfated to a degree of sulfation of from about 2.0 to about 3.0, and
wherein the at least one peptide(s) comprises the amino acid sequence of SEQ ID NO: 17.
2. The bioconjugate of claim 1 , wherein the chemically sulfated hyaluronic acid has a molecular weight of from about 150 to about 750 kDa.
3. The bioconjugate of claim 1 , wherein the chemically sulfated hyaluronic acid has a molecular weight of from about 200 to about 400 kDa.
4. The bioconjugate of claim 1 , wherein the collagen-binding unit of the peptide(s) bind to collagen with a dissociation constant (K d ) of less than about 1 mM.
5. The bioconjugate of claim 1 , wherein the peptide(s) comprise up to about 120 amino acids.
6. The bioconjugate of claim 1 , wherein the chemically sulfated hyaluronic acid does not contain oxidatively cleaved saccharide units.
7. A composition comprising the bioconjugate of claim 1 , wherein the average number of peptide(s) per chemically sulfated hyaluronic acid is from about 50 to about 150.
8. A composition comprising the bioconjugate of claim 1 , wherein the average number of peptide(s) per chemically sulfated hyaluronic acid is about 100.