Methods for making mixed allergen compositions
Methods of making mixed allergen compositions, e.g., substantially aerobic organism free mixed allergen compositions, and the resulting allergen composition, are provided.
1. A method of making a substantially aerobic organism free mixed allergen composition, comprising:
providing a mixed allergen composition comprising 6 to 20 allergens and a bulking agent, wherein the mixed allergen composition comprises at least 6% fat content;
milling the mixed allergen composition in a conical mill to obtain a milled composition with substantially consistent particle size; and
applying microwaves or radio frequency interference to the milled composition so that the milled composition is heated to at least 190° F. for at least 30 minutes,
thereby to obtain the substantially aerobic organism free mixed allergen composition;
wherein the mixed allergen composition comprises: at least one nut flour, wherein the at least one nut flour is selected from the group consisting of peanut flour, almond flour, walnut flour, cashew flour, hazelnut flour, pecan flour and pistachio flour; at least one fish powder, wherein the at least one fish powder is selected from the group consisting of cod powder and salmon powder; wheat; and powdered hen's egg or egg white.
2. The method of claim 1 , wherein the mixed allergen composition comprises at least 12% fat content.
3. The method of claim 1 , wherein milling the mixed allergen composition comprises using a rotor speed of about 9000 RPM.
4. The method of claim 1 , wherein milling the mixed allergen composition comprises passing the mixed allergen composition through a screen with an opening size of about 0.033 inches.
5. The method of claim 1 , wherein milling the mixed allergen composition comprises applying a vacuum suction through the conical mill.
6. The method of claim 1 , further comprising wetting the milled composition to a moisture content of about 6% to about 9% prior to applying microwaves or radio frequency interference to the milled mixed allergen composition.
7. The method of claim 6 , wherein the milled composition is wetted to a moisture content of about 9% prior to applying microwaves or radio frequency interference to the milled mixed allergen composition.
8. The method of claim 1 , further comprising wetting the milled composition to a water activity of about 0.65 to about 0.8 prior to applying microwaves or radio frequency interference to the milled mixed allergen composition.
9. The method of claim 1 , wherein the milled composition is heated to at least 200° F. for at least 60 minutes.
10. The method of claim 1 , wherein the milled composition is heated to no more than 250° F.
11. The method of claim 1 , wherein the milled composition is heated for no more than 360 minutes.
12. The method of claim 1 , wherein the milled composition is heated in a microwave blender.
13. The method of claim 1 , further comprising pressurizing the milled composition to at least 30 psi for at least 30 minutes.
14. The method of claim 1 , wherein the substantially aerobic organism free mixed allergen composition has fewer than about 4.5, about 4, about 3.5, about 3, about 2.5, about 2, or about 1.5 log CFU/g total aerobic organisms.
15. The method of claim 1 , wherein the substantially aerobic organism free mixed allergen composition has at least about 0.5, about 1, about 1.5, about 2, about 2.5, about 3, about 3.5, or about 4 log CFU/g fewer total aerobic organisms than the mixed allergen composition.
16. The method of claim 1 , wherein the substantially aerobic organism free mixed allergen composition has fewer than about 1 log CFU/g total coliforms.
17. The method of claim 1 , wherein the substantially aerobic organism free mixed allergen composition has a substantially similar protein structure as the mixed allergen composition as measured by SDS-PAGE.
18. The method of claim 1 , wherein the substantially aerobic organism free mixed allergen composition has a substantially similar allergen effect as the mixed allergen composition as measured by immune response in a patient.
19. The method of claim 1 , wherein the bulking agent comprises a sugar.
20. The method of claim 1 , wherein the mixed allergen composition further comprises at least one additional allergen component selected from the group consisting of oat flour, sesame seed flour, and shrimp flour.
21. The method of claim 1 , wherein the mixed allergen composition further comprises soy.
22. The method of claim 1 , wherein the mixed allergen composition comprises:
30 mg by protein weight shrimp powder;
30 mg by protein weight almond powder;
30 mg by protein weight wheat;
30 mg by protein weight cod powder; and
30 mg by protein weight powdered hen's egg.
23. A method of making a substantially aerobic organism free mixed allergen composition, comprising:
providing a mixed allergen composition comprising 6 to 20 allergens and a bulking agent, wherein the mixed allergen composition comprises at least 6% fat content;
milling the mixed allergen composition in a conical mill to obtain a milled composition with substantially consistent particle size, wherein milling the mixed allergen composition comprises applying a vacuum suction through the conical mill; and
applying microwaves or radio frequency interference to the milled composition so that the milled composition is heated to at least 190° F. for at least 30 minutes,
thereby to obtain the substantially aerobic organism free mixed allergen composition.
24. The method of claim 23 , wherein the substantially aerobic organism free mixed allergen composition has fewer than about 4.5, about 4, about 3.5, about 3, about 2.5, about 2, or about 1.5 log CFU/g total aerobic organisms.
25. The method of claim 23 , wherein the substantially aerobic organism free mixed allergen composition has at least about 0.5, about 1, about 1.5, about 2, about 2.5, about 3, about 3.5, or about 4 log CFU/g fewer total aerobic organisms than the mixed allergen composition.
26. The method of claim 23 , wherein the substantially aerobic organism free mixed allergen composition has fewer than about 1 log CFU/g total coliforms.
27. The method of claim 23 , wherein the substantially aerobic organism free mixed allergen composition has a substantially similar protein structure as the mixed allergen composition as measured by SDS-PAGE.
28. The method of claim 23 , wherein the substantially aerobic organism free mixed allergen composition has a substantially similar allergen effect as the mixed allergen composition as measured by immune response in a patient.