DNA vectors and elements for sustained gene expression in eukaryotic cells
The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel elements that further improve expression. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
1. A polynucleotide comprising an open reading frame encoding a glutamine synthetase fused to a heterologous C-terminal extension that reduces the activity of the glutamine synthetase, wherein the glutamine synthetase C-terminal extension comprises the amino acid sequence of SEQ ID NO:502.
2. The polynucleotide of claim 1 , wherein the glutamine synthetase comprises the amino acid sequence of any one of SEQ ID NOS:504-508.
3. The polynucleotide of claim 1 , wherein the glutamine synthetase is encoded by the nucleic acid sequence of any one of SEQ ID NOS:509-512.
4. The polynucleotide of claim 1 , wherein the glutamine synthetase fused to the heterologous C-terminal extension comprises the amino acid sequence of any one of SEQ ID NOS:513-518.
5. The polynucleotide of claim 1 , further comprising an intron with the nucleotide sequence of SEQ ID NO:141 in a 3′UTR.
6. The polynucleotide of claim 1 , wherein the glutamine synthetase open reading frame is operably linked to a promoter sequence that is 95% identical to the sequence of any one of SEQ ID NOS:83-90.
7. The polynucleotide of claim 1 , further comprising a 5′UTR between the promoter and the glutamine synthetase open reading frame.
8. The polynucleotide of claim 7 , wherein the 5′UTR comprises the sequence of any one of SEQ ID NOS:500-501.
9. The polynucleotide of claim 1 , wherein the polynucleotide comprises the sequence of any one of SEQ ID NOS:543-556.
10. The polynucleotide of claim 1 , wherein the polynucleotide further comprises a pair of transposon ITRs such that the open reading frame encoding the glutamine synthetase can be transposed by a transposase into the genome of a eukaryotic cell.
11. The polynucleotide of claim 1 , further comprising a promoter operably linked to a second open reading frame heterologous to the promoter, wherein the promoter comprises the sequence of any one of SEQ ID NOS:29-82.
12. The polynucleotide of claim 1 , further comprising an enhancer sequence that is at least 95% identical to the sequence of any one of SEQ ID NOS:11-28.
13. The polynucleotide of claim 1 , further comprising an intron sequence that is at least 95% identical to the sequence of any one of SEQ ID NOS:91-141.
14. The polynucleotide of claim 1 , further comprising a polyadenylation sequence that is at least 95% identical to the sequence of any one of SEQ ID NOS:192-227.
15. The polynucleotide of claim 1 , further comprising the sequence of any one of SEQ ID NOS:-142-191 or SEQ ID NOS:323-365.
16. The polynucleotide of claim 1 , further comprising a PRE element comprising the sequence of any one of SEQ ID NOS:242-316.
17. A method for expressing the polynucleotide of claim 1 comprising introducing the polynucleotide into a mammalian cell, wherein the glutamine synthetase is expressed.
18. A mammalian cell comprising the polynucleotide of claim 1 .