Devices and methods for characterization and control of biopolymers and synthetic polymers during manufacturing
Devices and methods for characterization and control of biopolymers and synthetic polymers during manufacturing. The device may include a body defining a fluid flow path. The body may be configured to receive a process flow liquid such that the process flow liquid may flow through an interior portion of the body. The body may also include a plurality of detectors inserted into said body, each of the plurality of detectors configured to monitor one or more process characteristics.
1. A device comprising:
a flow tube comprising an inner bore and an outer surface, the flow tube configured to be a first portion of a fluid flow path to receive a polymer containing solution in the inner bore and configured to permit light to pass to and from the inner bore to the outer surface,
wherein the flow tube comprises a first end configured to connect to a first tubing coupling and a second end configured to connect to a second tubing coupling,
wherein the first tubing coupling is configured to be a second portion of the fluid flow path to introduce the polymer containing solution into the flow tube, and the second tubing coupling is configured to be a third portion of the fluid flow path to provide an exit path of the polymer containing solution,
wherein the first end and the second end of the flow tube have inner and/or outer diameters matched to inner or outer diameters of the first tubing coupling and the second tubing coupling such that the flow tube is configured to be removably inserted into the fluid flow path of the polymer containing solution for continuously monitoring the polymer containing solution in real time without disturbing the flow of the polymer containing solution; and
an optical component tube comprising one or more optical detectors configured to monitor one or more process characteristics, the optical component tube having a body configured to receive the flow tube, wherein the flow tube is configured to be releasably attached to the body of the optical component tube.
2. The device according to claim 1 , wherein the flow tube contains one or more windows permitting light to pass from the inner bore to the outer surface of the flow tube.
3. The device according to claim 1 , wherein the flow tube comprises a transparent material.
4. The device according to claim 1 , wherein the flow tube comprises a disposable material and/or a sterilizeable material.
5. The device according to claim 1 , wherein the optical component tube with the flow tube inside it is configured to be immersed in a biopolymer processing vessel without the optical containing tube making contact with the liquid contents or the vessel.
6. The device according to claim 5 further comprising one or more lens assemblies configured to manipulate the beam produced by the one or more light sources.
7. The device according to claim 1 , wherein the optical component tube comprises one or more light sources.
8. The device according to claim 7 , further comprising one or more beam dumps or beam stops.
9. The device according to claim 1 , further comprising a computing and analysis device, communicatively coupled with the one or more optical detectors, the computing and analysis device configured to determine one or more process characteristics of the polymer containing liquid.
10. The device according to claim 1 , wherein the polymer containing liquid is a biopolymer solution, and the one or more detectors are configured to monitor one or more of Mw, Rg, concentration of the biopolymer, unfolding of the biopolymer in time, change of molecular weight of the biopolymer in time, conformational change of the biopolymer, total solution viscosity, biopolymer intrinsic viscosity, degradation or aggregation, rate of aggregation or degradation of the biopolymer, mechanism causing the molecular weight of the biopolymer to change, number concentration of subvisible particles, early detection of changes in molecular weight, presence and of anisotropic or large particles, number concentration of anisotropic or large particles, and the fraction of biopolymer mass in aggregated form.
11. The device according to claim 1 , wherein the one or more process characteristics is selected from the group consisting of concentration of the biopolymer, unfolding of the biopolymer in time, change of molecular weight of the biopolymer in time, degradation or aggregation, rate of aggregation or degradation of the biopolymer, mechanism causing the molecular weight of the biopolymer to change, number concentration of subvisible particles, early detection of changes in molecular weight, total solution viscosity, biopolymer intrinsic viscosity, biopolymer radius of gyration, and the fraction of biopolymer mass in aggregated form.
12. The device according to claim 1 , wherein the one or more detectors is selected from the group consisting of a single angle or a multiple angle light scattering (MALS) detector, a depolarized scattering detector, a particle counter, a dynamic light scattering (DLS) detector, an ultraviolet (UV) absorption detector, a fluorescence detector, and a viscosity detector.
13. The device according to claim 1 , wherein the optical component tube comprises modular ring detectors configured to slide over the flow tube and are releasably attached to the flow tube.
14. The device according to claim 1 , further comprising one or more scattering light detection fibers.
15. The device according to claim 1 , further comprising one or more band pass filters.
16. The device according to claim 1 , wherein the detectors are configured to share the same flow path without any intervening fluid fittings or connector pieces, and wherein there is no sample chamber.
17. The device according to claim 1 , wherein the flow tube can be inserted into the fluid flow path via the first tubing coupling and second tubing coupling or removed from the fluid flow path.
18. The device according to claim 1 , wherein the flow tube is a disposable insert.
19. The device according to claim 1 , wherein the optical component tube comprises a plurality of modular detector rings and a plurality of releasable connections that allow the plurality of modular detector rings to interlock or attach sufficiently to provide an integrated fluid path.
20. The device according to claim 19 , wherein the plurality of modular detector rings is configured for measurements along the flow path.
21. The device according to claim 1 , wherein the polymer containing solution is a diluted stream.
22. The device according to claim 1 , wherein the polymer containing solution in the fluid flow path is a production stream for the production of biopolymers and synthetic polymers, whereby the polymer containing solution in the fluid flow path is not removed from a production batch.
23. A device comprising:
a body having a proximal end and a distal end spaced from the proximal end; the body comprising:
a flow tube having an outer surface and an inner surface, the inner surface defining a passageway extending through the flow tube from the proximal end to the distal end of the body, the passageway comprising a first portion of a fluid flow path configured to receive a polymer containing solution and to permit the exit of the polymer containing solution, where the flow tube is configured to permit light to pass through the flow tube to and from the passageway,
wherein the flow tube is configured such that the passageway is dimensioned to match an inner diameter of a first tube coupled to the proximal end, a second tube coupled to the distal end, or a combination thereof,
wherein the first tube is configured to be a second portion of the fluid flow path to introduce the polymer containing solution into the body, and the second tube is configured to be a third portion of the fluid flow path to provide an exit path of the polymer containing solution,
wherein the flow tube is configured to be removably inserted into the fluid flow path of the polymer containing solution for continuously monitoring the polymer containing solution in real time without disturbing the flow of the polymer containing solution; and
an optical component tube having an inner surface defining an inner bore configured to receive the flow tube and comprising one or more optical detectors configured to detect one or more process characteristics of the polymer containing solution within the flow tube without diverting any of the fluid flow,
wherein the flow tube is configured to be releasably attached to the inner bore of the optical opponent tube.
24. The device according to claim 23 , wherein the flow tube can be inserted into the fluid flow path via the first tubing coupling and second tubing coupling or removed from the fluid flow path.
25. The device according to claim 23 , wherein the flow tube is a disposable insert.
26. The device according to claim 23 , wherein the optical component tube comprises a plurality of modular detector rings and a plurality of releasable connections that allow the plurality of modular detector rings to interlock or attach sufficiently to provide an integrated fluid path.
27. The device according to claim 26 , wherein the plurality of modular detector rings is configured for measurements along the flow path.