Devices and methods for processing fluid samples
Described are devices for and methods of modulating a fluid sample. The devices ( 10, 40, 60, 80, 100, 130, 160, 220 ) include at least one sample-modulating component ( 20, 76, 78, 90, 110, 112, 116, 150, 152, 154, 162, 164, 182, 184, 186, 222 , and 230 ) and, in some embodiments, two or more sample-modulating components. The sample-modulating components are each capable of performing a function selected from the following group: concentrating the sample to increase a concentration of a first constituent of the sample; diluting the sample to decrease a concentration of a second constituent in of the sample; desalinating the sample to decrease the total moles of salt in the sample volume or causing a temporary decrease in the osmolarity; adjusting pH of the sample to bring a pH of the sample into a predetermined range; absorbing one or more nonpolar substances to decrease a concentration of the nonpolar substances; and delivering one or more reagents to the sample to provide a desired concentration of the reagent in the sample.
1. A device for preparing a fluid sample comprising:
a sample-modulating component capable of delivering one or more reagents to the fluid sample by osmotic flux to provide a desired concentration of the one or more reagents in the sample, the sample-modulating component comprising a membrane separating a reservoir from a channel, the reservoir configured to house the one or more reagents, and the membrane configured to allow passage of the one or more reagents from the reservoir to the channel, wherein the osmotic flux includes transporting the one or more reagents from the reservoir to the channel by way of an applied pressure on the one or more reagents; and
an analyte-specific sensor for measuring an analyte in the sample.
2. The device of claim 1 , wherein the one or more reagents include at least two solutes, the at least two solutes configured to be delivered to the sample at a ratio, and the ratio of the at least two solutes is measured to give a quantitative measurement.
3. A method of processing a fluid sample comprising:
collecting the fluid sample;
flowing the fluid sample through a sample-modulating component, the sample modulating component comprising a reservoir, a channel, and a membrane separating the reservoir from the channel;
delivering one or more reagents, housed in the reservoir, to the sample by osmotic flux by applying a pressure on the one or more reagents to provide a desired concentration of the one or more reagents in the sample to form a modulated sample; and
measuring an analyte in the modulated sample using an analyte-specific sensor.