RNA nanostructures and methods of making and using RNA nanostructures
Certain embodiments provide RNA nanostructure (e.g., comprising one single-stranded RNA (ssRNA) molecule, wherein the RNA nanostructure comprises at least one paranemic cohesion crossover), as well as compositions and methods of use thereof. In certain embodiments, such RNA nanostructures are immuno-modulatory (e.g., immuno-stimulatory).
1. An RNA nanostructure comprising at least one single-stranded RNA (ssRNA) molecule,
wherein the RNA nanostructure comprises at least two structural repeating units of 33 nucleotides in length, and wherein each structural repeating unit comprises, in order: a first region of a double helix 8 nucleotides in length, a first paranemic cohesion crossover 8 nucleotides in length, a second region of a double helix 9 nucleotides in length, and a second paranemic cohesion crossover 8 nucleotides in length.
2. An RNA nanostructure comprising a nucleic acid sequence having at least about 75% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12 or SEQ ID NO:13.
3. The RNA nanostructure of claim 2 , wherein the nucleic acid sequence has at least about 85% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10 or SEQ ID NO:11, SEQ ID NO:12 or SEQ ID NO:13.
4. The RNA nanostructure of claim 2 , wherein the nucleic acid sequence has at least about 95% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10 or SEQ ID NO:11, SEQ ID NO:12 or SEQ ID NO:13.
5. The RNA nanostructure of claim 2 wherein the nucleic acid sequence has at least about 99% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10 or SEQ ID NO:11, SEQ ID NO:12 or SEQ ID NO:13.
6. The RNA nanostructure of claim 2 , comprising SEQ ID NO:1.
7. The RNA nanostructure of claim 2 , consisting of SEQ ID NO:1.
8. The RNA nanostructure of any one of claims 1 - 7 , wherein the RNA nanostructure has rectangular, diamond, triangle, or tetrahedron shape.
9. A nucleic acid having at least about 75% identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12 or SEQ ID NO:13.
10. The nucleic acid of claim 9 , wherein the nucleic acid has at least about 90% identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12 or SEQ ID NO:13.