Engineered shell proteins for microcompartment shell electron transfer and catalysis
View Patent ↗Described herein are bacterial microcompartments shell proteins modified to stably incorporate iron-sulfur clusters. Such bacterial microcompartments shell proteins exhibit redox cycling and confer electron transfer functionality to bacterial microcompartment shells.
1. A polypeptide comprising at least 95% sequence identity to SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, or SEQ ID NO:8, and comprising a cysteine substitution at position 55, 56 or at both positions 55 and 56.
2. A host cell comprising the polypeptide of claim 1 .
3. A bacterial microcompartment comprising the polypeptide of claim 1 .
4. The bacterial microcompartment of claim 3 , further comprising at least one BMC-H subunit.
5. The bacterial microcompartment of claim 4 , further comprising an enzyme.
6. The bacterial microcompartment of claim 5 , wherein the enzyme catalyzes an oxidation reaction.
7. The bacterial microcompartment of claim 3 , further comprising at least one BMC-P subunit.
8. The bacterial microcompartment of claim 3 , further comprising one or more BMC-T2 proteins.
9. The bacterial microcompartment of claim 3 , further comprising one or more BMC-T3 proteins.
10. The bacterial microcompartment of claim 3 , further comprising one or more iron atoms.
11. The bacterial microcompartment of claim 3 , further comprising one or more [4Fe-4S] clusters.
12. The bacterial microcompartment of claim 3 , further comprising an enzyme that can catalyze the conversion of NAD/NADP.
13. A host cell comprising the bacterial microcompartment of claim 3 .
14. The polypeptide of claim 1 wherein the polypeptide comprises a substitution of an amino acid at position 56 or position 154.
15. The polypeptide of claim 1 wherein the polypeptide comprises a substitution of an amino acid at position 56 and position 154.
16. The polypeptide of claim 1 wherein the polypeptide comprises a substitution of a serine at position 56 with a cysteine (S56C).
17. The polypeptide of claim 1 wherein the polypeptide comprises a substitution of an isoleucine at position 154 with a phenylalanine (I154F).