Modulation of expression of acyltransferases to modify hydroxycinnamic acid content
The invention provides methods of engineering plants to modulate hydroxycinnamic acid content. The invention additionally provides compositions and methods comprising such plants.
1. A method of engineering a plant to increase the ferulic acid content in the plant, the method comprising:
introducing an expression cassette into the plant, wherein the expression cassette comprises a polynucleotide encoding an AT5 acyltransferase, wherein the acyltransferase has at least 95% identity to SEQ ID NO:8, and culturing the plant under conditions in which the acyltransferase is expressed.
2. The method of claim 1 , wherein the acyltransferase has at least 99% identity to SEQ ID N0:8.
3. The method of claim 2 , wherein the acyltransferase comprises SEQ ID NO:8.
4. The method of claim 1 , wherein the polynucleotide is operably linked to a promoter endogenous to the plant.
5. The method of claim 1 , wherein the expression cassette comprises a promoter to which the polynucleotide is operably linked.
6. The method of claim 5 , wherein the promoter is a tissue-specific promoter.
7. The method of claim 1 , wherein the polynucleotide has at least 95% identity to SEQ ID NO:7.
8. The method of claim 7 , wherein the polynucleotide has at least 99% identity to SEQ ID NO:7.
9. The method of claim 8 , wherein the polynucleotide comprises SEQ ID NO:7.
10. The method of claim 1 , wherein the plant is a grass plant.
11. The method of claim 10 , wherein the grass plant is rice, corn, switchgrass, sorghum, millet, miscanthus, sugarcane, alfalfa, wheat, soy, rye, barley, turfgrass, hemp, bamboo, rape, sunflower, or brachypodium.
12. A plant engineered by the method of claim 1 , or a progeny of the plant.
13. A plant cell comprising a polynucleotide encoding an AT5 operably linked to a heterologous promoter; or a plant comprising a heterologous polynucleotide encoding an AT5 protein.
14. Biomass comprising the plant or a part of the plant of claim 12 .
15. A method of obtaining an increased amount of soluble sugars from a plant in a saccharification reaction, the method comprising:
subjecting the plant of claim 12 to a saccharification reaction, thereby increasing the amount of soluble sugars that can be obtained from the plant as compared to a wild-type plant.