Methods of detection and removal of rhabdoviruses from cell lines
The present disclosure relates to compositions, methods, mixtures, and kits for detecting the presence of, and for removing, a virus from a product produced in an insect cell. The disclosure also relates to proteins, peptides, polypeptides, drug substances, biological products, vaccine antigens, and virus-like particles that are produced in an insect cell and that are free or substantially free of a virus. The disclosure also relates to compositions, methods, assays, and kits for detecting a rhabdovirus in a sample.
1. A rhabdovirus-free Sf9 or Sf21 insect cell comprising a biological product.
2. The insect cell of claim 1 , wherein the insect cell is free of a nucleotide sequence having at least 70% homology to SEQ ID NO:1.
3. The insect cell of claim 1 , wherein the insect cell is an Sf9 cell.
4. The insect cell of claim 3 , wherein the Sf9 cell is derived from the cell line deposited as ATCC CRL-1711.
5. The insect cell of claim 1 , wherein the biological product is a protein, peptide, drug substance, virus-like particle (VLP), DNA, RNA, virus, or vaccine antigen.
6. The insect cell of claim 1 , wherein the biological product is a recombinant virus for gene therapy.
7. The insect cell of claim 1 , wherein the biological product is a protein or virus-like particle (VLP).
8. The insect cell of claim 1 , wherein the insect cell is free of a nucleotide sequence comprising the sequence according to SEQ ID NO: 1.