2-dimensional surfaces capable of monitoring stimuli-responsive behavior and methods of use thereof
View Patent ↗2-dimensional surfaces modified with elastin-like polymer (ELP) that transitions between states in the presence of one or more analytes are described. This transition can be monitored to identify the presence and concentration of the one or more analytes. 2-dimensional surfaces of the present disclosure may also or alternatively be configured such that when an analyte binds to ELP on the 2-dimensional surface, such binding changes a degree at which the ELP responds to various non-analyte stimuli (e.g., pH, temperature, etc.). In such examples, the 2-dimensional surface may detect the presence of analyte based on the how the ELP responds to the non-analyte stimuli. For example, ELP on a 2-dimensional surface may transition between states at a first temperature when analyte is not present (e.g., bound to the ELP). The temperature at which the ELP transitions may change based on how much analyte (e.g., what concentration of analyte) is present.
1. A 2-dimensional metal surface, comprising:
a plurality of elastin-like polymers (ELPs), each ELP covalently attached to the 2-dimensional metal surface and covalently attached to at least one biorecognition element, wherein the ELPs are configured to transition between at least two conformational states in the absence of in-solution ELPs.
2. The 2-dimensional metal surface of claim 1 , wherein the ELPs are configured as part of a monolayer.
3. The 2-dimensional metal surface of claim 1 , wherein the biorecognition element is configured to bind at least one bioanalyte.
4. The 2-dimensional metal surface of claim 3 , wherein the ELPs are configured to have an altered stimulus response behavior when the biorecognition element is bound to the bioanalyte.
5. The 2-dimensional metal surface of claim 4 , wherein the stimulus response behavior is a temperature response behavior.
6. The 2-dimensional metal surface of claim 4 , wherein the stimulus response behavior is an ionic strength response behavior.
7. The 2-dimensional metal surface of claim 4 , wherein the stimulus response behavior is a pH response behavior.
8. The 2-dimensional metal surface of claim 4 , wherein the stimulus response behavior is an electric field response behavior.
9. The 2-dimensional metal surface of claim 1 , wherein the ELPs are configured with one or more electrochemical tags.
10. The 2-dimensional metal surface of claim 1 , wherein the at least two conformational states comprise an elongated state and a collapsed state of the ELP.
11. The 2-dimensional metal surface of claim 1 , wherein the biorecognition element is a protein.
12. The 2-dimensional metal surface of claim 1 , wherein the ELPs are configured to transition between the at least two conformational states in response to ligand binding to the biorecognition element.
13. The 2-dimensional metal surface of claim 1 , wherein the 2-dimensional metal surface comprises gold.
14. The 2-dimensional metal surface of claim 1 , wherein the covalently-attached ELPs are the only ELPs in a system that comprises the 2-dimensional metal surface.
15. The 2-dimensional metal surface of claim 1 , wherein the covalently-attached ELPs are end-tethered to the 2-dimensional metal surface.
16. An electrochemical sensor comprising the 2-dimensional metal surface of claim 1 .
17. The electrochemical sensor of claim 16 , wherein the electrochemical sensor further comprises an electrode.
18. The electrochemical sensor of claim 17 , wherein the sensor is configured to have an altered stimulus response behavior that can be detected with an electrochemical detection technique when the biorecognition element is bound to the bioanalyte.
19. A device comprising the 2-dimensional metal surface of claim 1 .
20. An electrode comprising the 2-dimensional metal surface of claim 1 .
21. A 2-dimensional metal surface, comprising:
a plurality of elastin-like polymers (ELPs), each ELP covalently attached to a biorecognition element and the 2-dimensional metal surface, wherein the biorecognition element is configured to bind at least one bioanalyte and alter the conformational state of the ELPs based on the binding of the bioanalyte in the absence of in-solution ELPs.
22. The 2-dimensional metal surface of claim 21 , wherein the ELPs are configured as part of a monolayer.
23. A method of using a 2-dimensional metal surface capable of performing a stimulus-response interaction, comprising:
contacting a sample with the 2-dimensional metal surface comprising a plurality of elastin-like polymers (ELPs), each ELP covalently attached to the 2-dimensional metal surface and covalently attached to at least one biorecognition element, wherein the ELPs transition between at least two conformational states in response to the ELPs being contacted with at least one stimulus in the absence of in-solution ELPs; and
monitoring a state transition of the sample-contacted ELPs using at least one electrochemical detection technique capable of detecting the state transition.
24. The method of claim 23 , wherein the monitored state transition of the sample-contacted ELPs is compared to a control state of the ELPs not contacted with the at least one stimulus, and a difference detected between the monitored state transition and the control state indicates the presence of the at least one stimulus in the sample.