Granted Patent B2

US 12,514,957 · App. 16/686,158 · Granted Jan 6, 2026

Inventors: Tal Dvir (LeHavim, IL); Dan Peer (Kiryat-Ono, IL); Michal Shevach (Tel-Aviv, IL); Neta Soffer Tsur (Zikhron-Yaakov, IL)

Assignee: Ramot at Tel-Aviv University Ltd.

A61L27/3633A61K35/34A61K35/44A61L27/14A61L27/3604A61L27/3687A61L27/3804A61L27/3826A61L27/3834A61L27/52A61L27/58C12N5/0657C12N5/0691C12N5/0696A61L2430/20A61L2430/40

Abstract

Claims (13)

1 . A method of 3D printing a 3D structure of cardiac tissue, the method comprising:

(a) digesting decellularized omentum with a proteolytic enzyme to generate a liquid composition which comprises solubilized, decellularized omentum, said liquid composition being capable of forming a gel at 37° C.;

wherein the liquid composition is more than 99% devoid of nucleic acid or residual nucleic acid components and wherein the liquid composition is more than 99% devoid of lipid components;

(b) combining said liquid composition with viable cardiac cells to form a mixture comprising solubilized, decellularized omentum and the viable cardiac cells; and

(c) extruding said mixture through an aperture; and

(d) generating 3D structure of cardiac tissue comprising viable cardiac cells having an elongated morphology and decellularized omentum, thereby 3D printing the 3D structure of cardiac tissue;

further comprising decellularizing said omentum prior to said digesting wherein said decellularizing comprises:

(i) exposing the omentum to a hypotonic solution to produce hypotonic shock; (ii) exposing the omentum to freeze-thaw conditions following step (i); (iii) dehydrating said omentum following step (ii); (iv) extracting fat from the dehydrated omentum using polar and non-polar extraction agents following step (iii); (v) rehydrating the dehydrated omentum following step (iv); and (vi) extracting cells from said rehydrated omentum following step (v); wherein step (iv) also comprises washing the dehydrated omentum multiple times with acetone, followed by three incubations in a 60:40 hexane:acetone solution.

2 . The method of claim 1 , wherein said omentum is human omentum.

3 . The method of claim 1 , wherein a diameter of said aperture is between 0.1-0.7 mm.

4 . The method of claim 1 , wherein the mixture consists of viable cells and decellularized omentum.

5 . The method of claim 1 , further comprising combining said liquid composition with endothelial cells following step (a) and prior to step (c).

6 . The method of claim 5 , wherein said 3D structure of cardiac tissue comprises blood vessels.

Assignments (1)

Continuity (5)

References Cited (135)